| PAZOUREK, Jiří a Karel ŠMEJKAL. Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica. Molecules. BASEL: Mayer und Muller, 2016, roč. 21, č. 11, s. 1-11. ISSN 1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules21111495. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1726831,
author = {Pazourek, Jiří and Šmejkal, Karel},
article_location = {BASEL},
article_number = {11},
doi = {http://dx.doi.org/10.3390/molecules21111495},
keywords = {fraction collection; evaporative light scattering detector; hydrophilic interaction liquid chromatography; mixed-mode chromatography; Pectinatella magnifica},
language = {eng},
issn = {1420-3049},
journal = {Molecules},
title = {Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica},
url = {https://www.mdpi.com/1420-3049/21/11/1495},
volume = {21},
year = {2016}
}
TY - JOUR
ID - 1726831
AU - Pazourek, Jiří - Šmejkal, Karel
PY - 2016
TI - Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica
JF - Molecules
VL - 21
IS - 11
SP - 1-11
EP - 1-11
PB - Mayer und Muller
SN - 14203049
KW - fraction collection
KW - evaporative light scattering detector
KW - hydrophilic interaction liquid chromatography
KW - mixed-mode chromatography
KW - Pectinatella magnifica
UR - https://www.mdpi.com/1420-3049/21/11/1495
N2 - The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-beta-D-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.
ER -
PAZOUREK, Jiří a Karel ŠMEJKAL. Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica. \textit{Molecules}. BASEL: Mayer und Muller, 2016, roč.~21, č.~11, s.~1-11. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules21111495.
|
