Prenylated flavonoid morusin protects against TNBS-induced
colitis in rats

J 2017

Prenylated flavonoid morusin protects against TNBS-induced colitis in rats

VOCHYANOVA, Z; Marie ČULENOVÁ; Dominik ROTREKL; V SMEKAL; P FICTUM et al.

Základní údaje

Originální název

Prenylated flavonoid morusin protects against TNBS-induced colitis in rats

Autoři

VOCHYANOVA, Z; Marie ČULENOVÁ; Dominik ROTREKL; V SMEKAL; P FICTUM; Pavel SUCHÝ; Jan GAJDZIOK; Karel ŠMEJKAL a J HOSEK

Vydání

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.766

Označené pro přenos do RIV

Organizační jednotka

Farmaceutická fakulta

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2021 07:56, Mgr. Hana Hurtová

Anotace

V originále

Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitroben-zensulfonic acid in Wistar rats and to compare it with sulfasalazine, a drug conventionally used in the treatment of inflammatory bowel disease. Morusin was administered by gavage at doses of 12.5, 25, or 50 mg/kg/day for five days. The colonic tissue was evaluated macroscopically, histologically, and by performing immunodetection and zymographic analysis to determine the levels of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], interleukin (IL)-1 beta, and transforming growth factor (TGF)-beta 1 and the activities of matrix metalloproteinases (MMP) 2 and 9. The tissue damage scores were significantly reduced with increasing dose of morusin, however efficacy was not demonstrated at the highest dose. At the dose of 12.5 mg/kg, morusin exerted therapeutic effectivity similar to that of sulfasalazine (50 mg/kg). This was associated with significant reduction of TGF-beta 1 levels and MMP2 and MMP9 activities, and slight reduction of IL-1 beta. Our results suggest that morusin possesses therapeutic potential for the treatment of chronic inflammatory diseases.

Citovat

VOCHYANOVA, Z; Marie ČULENOVÁ; Dominik ROTREKL; V SMEKAL; P FICTUM; Pavel SUCHÝ; Jan GAJDZIOK; Karel ŠMEJKAL a J HOSEK. Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. Plos one. San Francisco: Public Library of Science, 2017, roč. 12, č. 8, 14 s. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0182464.

@article{1726859,
   author = {Vochyanova, Z and Čulenová, Marie and Rotrekl, Dominik and Smekal, V and Fictum, P and Suchý, Pavel and Gajdziok, Jan and Šmejkal, Karel and Hosek, J},
   article_location = {San Francisco},
   article_number = {8},
   doi = {https://doi.org/10.1371/journal.pone.0182464},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Prenylated flavonoid morusin protects against TNBS-induced colitis in rats},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1726859
AU  - Vochyanova, Z - Čulenová, Marie - Rotrekl, Dominik - Smekal, V - Fictum, P - Suchý, Pavel - Gajdziok, Jan - Šmejkal, Karel - Hosek, J
PY  - 2017
TI  - Prenylated flavonoid morusin protects against TNBS-induced colitis in rats
JF  - Plos one
VL  - 12
IS  - 8
PB  - Public Library of Science
SN  - 19326203
N2  - Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitroben-zensulfonic acid in Wistar rats and to compare it with sulfasalazine, a drug conventionally used in the treatment of inflammatory bowel disease. Morusin was administered by gavage at doses of 12.5, 25, or 50 mg/kg/day for five days. The colonic tissue was evaluated macroscopically, histologically, and by performing immunodetection and zymographic analysis to determine the levels of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], interleukin (IL)-1 beta, and transforming growth factor (TGF)-beta 1 and the activities of matrix metalloproteinases (MMP) 2 and 9. The tissue damage scores were significantly reduced with increasing dose of morusin, however efficacy was not demonstrated at the highest dose. At the dose of 12.5 mg/kg, morusin exerted therapeutic effectivity similar to that of sulfasalazine (50 mg/kg). This was associated with significant reduction of TGF-beta 1 levels and MMP2 and MMP9 activities, and slight reduction of IL-1 beta. Our results suggest that morusin possesses therapeutic potential for the treatment of chronic inflammatory diseases.
ER  -

VOCHYANOVA, Z; Marie ČULENOVÁ; Dominik ROTREKL; V SMEKAL; P FICTUM; Pavel SUCHÝ; Jan GAJDZIOK; Karel ŠMEJKAL a J HOSEK. Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. \textit{Plos one}. San Francisco: Public Library of Science, 2017, roč.~12, č.~8, 14 s. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0182464.

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