| PAZOUREK, Jiří. Determination of glucosamine and monitoring of its mutarotation by hydrophilic interaction liquid chromatography with evaporative light scattering detector. Biomedical Chromatography. Wiley, 2018, roč. 32, č. 12, s. 1-8. ISSN 1099-0801. Dostupné z: https://dx.doi.org/10.1002/bmc.4368. |
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@article{1726897,
author = {Pazourek, Jiří},
article_number = {12},
doi = {http://dx.doi.org/10.1002/bmc.4368},
keywords = {calibration; ELSD; glucosamine; HILIC; mutarotation},
language = {eng},
issn = {1099-0801},
journal = {Biomedical Chromatography},
title = {Determination of glucosamine and monitoring of its mutarotation by hydrophilic interaction liquid chromatography with evaporative light scattering detector},
url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4368},
volume = {32},
year = {2018}
}
TY - JOUR
ID - 1726897
AU - Pazourek, Jiří
PY - 2018
TI - Determination of glucosamine and monitoring of its mutarotation by hydrophilic interaction liquid chromatography with evaporative light scattering detector
JF - Biomedical Chromatography
VL - 32
IS - 12
SP - 1-8
EP - 1-8
PB - Wiley
SN - 10990801
KW - calibration
KW - ELSD
KW - glucosamine
KW - HILIC
KW - mutarotation
UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4368
N2 - Saccharides and their derivatives are typical polar analytes without a suitable UV‐chromophore that are nowadays analyzed by HPLC (high‐performance liquid chromatography) under HILIC (hydrophilic interaction liquid chromatography) mode. Usually an evaporative light scattering detector (ELSD) is utilized which, however, gives a nonlinear response. A procedure to overcome the problem of mutarotating (time‐varying) analytes recorded with such a nonlinear response detector is described. The procedure was applied for determination of glucosamine in two commercially available pharmaceutical formulations containing the common inorganic ions that the detector gives a response to. Under optimized conditions, both the anomers of glucosamine were separated and could be determined separately. Owing to the short retention time of the analyte (a run time <4 min) and relatively slow kinetics of the anomeric conversion (equilibration time 2.5 h), mutarotation could be monitored and corresponding rate constants calculated.
ER -
PAZOUREK, Jiří. Determination of glucosamine and monitoring of its mutarotation by hydrophilic interaction liquid chromatography with evaporative light scattering detector. \textit{Biomedical Chromatography}. Wiley, 2018, roč.~32, č.~12, s.~1-8. ISSN~1099-0801. Dostupné z: https://dx.doi.org/10.1002/bmc.4368.
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