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@article{1727077, author = {Ondrejickova, P. and Sturdikova, M. and Hushegyi, A. and Švajdlenka, Emil and Markosova, K. and Certik, M.}, article_location = {HEIDELBERG}, article_number = {9}, doi = {http://dx.doi.org/10.1007/s10295-016-1800-4}, keywords = {Streptomyces sp.; Isoflavone aglycones; Atomic force microscopy; Antimycin}, language = {eng}, issn = {1367-5435}, journal = {JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY}, title = {Endophytic Streptomyces sp AC35, a producer of bioactive isoflavone aglycones and antimycins}, volume = {43}, year = {2016} }
TY - JOUR ID - 1727077 AU - Ondrejickova, P. - Sturdikova, M. - Hushegyi, A. - Švajdlenka, Emil - Markosova, K. - Certik, M. PY - 2016 TI - Endophytic Streptomyces sp AC35, a producer of bioactive isoflavone aglycones and antimycins JF - JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY VL - 43 IS - 9 SP - 1333-1344 EP - 1333-1344 PB - SPRINGER HEIDELBERG SN - 13675435 KW - Streptomyces sp. KW - Isoflavone aglycones KW - Atomic force microscopy KW - Antimycin N2 - In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatographymass spectrometry (LC-MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds. ER -
ONDREJICKOVA, P., M. STURDIKOVA, A. HUSHEGYI, Emil ŠVAJDLENKA, K. MARKOSOVA a M. CERTIK. Endophytic Streptomyces sp AC35, a producer of bioactive isoflavone aglycones and antimycins. \textit{JOURNAL OF INDUSTRIAL MICROBIOLOGY \&{}amp; BIOTECHNOLOGY}. HEIDELBERG: SPRINGER HEIDELBERG, 2016, roč.~43, č.~9, s.~1333-1344. ISSN~1367-5435. Dostupné z: https://dx.doi.org/10.1007/s10295-016-1800-4.
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