Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit
Antiviral Activity against HSV-2 and Anti-Enzymatic Properties
against Urease by an ESI-MS Based Assay

J 2017

Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-Enzymatic Properties against Urease by an ESI-MS Based Assay

HASSAN, STS.; Emil ŠVAJDLENKA a K. BERCHOVA-BIMOVA

Základní údaje

Originální název

Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-Enzymatic Properties against Urease by an ESI-MS Based Assay

Autoři

HASSAN, STS.; Emil ŠVAJDLENKA a K. BERCHOVA-BIMOVA

Vydání

Molecules, BASEL, Mayer und Muller, 2017, 1420-3049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.098

Označené pro přenos do RIV

DOI

https://doi.org/10.3390/molecules22050722

UT WoS

000404522900045

Klíčová slova anglicky

anti-HSV-2 activity; ESI-mass spectrometry-based assay; urease inhibitors; protocatechuic acid; Hibiscus sabdariffa L.; bacterial infection

Změněno: 24. 2. 2021 14:19, PharmDr. Jitka Michlíčková

Anotace

V originále

For decades, Hibiscus sabdariffa L. and its phytochemicals have been shown to possess a wide range of pharmacologic properties. In this study, aqueous extract of Hibiscus sabdariffa (AEHS) and its bioactive constituent protocatechuic acid (PCA), have been evaluated in vitro for their antiviral activity against HSV-2 clinical isolates and anti-enzymatic activity against urease. Antiherpetic activity was evaluated by the titer reduction assay in infected Vero cells, and cytotoxicity was evaluated by the neutral red dye-uptake method. Anti-urease activity was determined by a developed Electrospray Ionization-Mass Spectrometry (ESI-MS)-based assay. PCA showed potent anti-HSV-2 activity compared with that of acyclovir, with EC50 values of 0.92 and 1.43 mu g.mL(-1), respectively, and selectivity indices > 217 and > 140, respectively. For the first time, AEHS was shown to exert anti-urease inhibition activity, with an IC50 value of 82.4 mu g.mL(-1). This, combined with its safety, could facilitate its use in practical applications as a natural urease inhibitor. Our results present Hibiscus sabdariffa L. and its bioactive compound PCA as potential therapeutic agents in the treatment of HSV-2 infection and the treatment of diseases caused by urease-producing bacteria.

Citovat

HASSAN, STS.; Emil ŠVAJDLENKA a K. BERCHOVA-BIMOVA. Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-Enzymatic Properties against Urease by an ESI-MS Based Assay. Molecules. BASEL: Mayer und Muller, 2017, roč. 22, č. 5, 12 s. ISSN 1420-3049. Dostupné z: https://doi.org/10.3390/molecules22050722.

@article{1727092,
   author = {Hassan, STS. and Švajdlenka, Emil and BerchovaandBimova, K.},
   article_location = {BASEL},
   article_number = {5},
   doi = {https://doi.org/10.3390/molecules22050722},
   keywords = {anti-HSV-2 activity; ESI-mass spectrometry-based assay; urease inhibitors; protocatechuic acid; Hibiscus sabdariffa L.; bacterial infection},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-Enzymatic Properties against Urease by an ESI-MS Based Assay},
   volume = {22},
   year = {2017}
}

TY  - JOUR
ID  - 1727092
AU  - Hassan, STS. - Švajdlenka, Emil - Berchova-Bimova, K.
PY  - 2017
TI  - Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-Enzymatic Properties against Urease by an ESI-MS Based Assay
JF  - Molecules
VL  - 22
IS  - 5
PB  - Mayer und Muller
SN  - 14203049
KW  - anti-HSV-2 activity
KW  - ESI-mass spectrometry-based assay
KW  - urease inhibitors
KW  - protocatechuic acid
KW  - Hibiscus sabdariffa L.
KW  - bacterial infection
N2  - For decades, Hibiscus sabdariffa L. and its phytochemicals have been shown to possess a wide range of pharmacologic properties. In this study, aqueous extract of Hibiscus sabdariffa (AEHS) and its bioactive constituent protocatechuic acid (PCA), have been evaluated in vitro for their antiviral activity against HSV-2 clinical isolates and anti-enzymatic activity against urease. Antiherpetic activity was evaluated by the titer reduction assay in infected Vero cells, and cytotoxicity was evaluated by the neutral red dye-uptake method. Anti-urease activity was determined by a developed Electrospray Ionization-Mass Spectrometry (ESI-MS)-based assay. PCA showed potent anti-HSV-2 activity compared with that of acyclovir, with EC50 values of 0.92 and 1.43 mu g.mL(-1), respectively, and selectivity indices > 217 and > 140, respectively. For the first time, AEHS was shown to exert anti-urease inhibition activity, with an IC50 value of 82.4 mu g.mL(-1). This, combined with its safety, could facilitate its use in practical applications as a natural urease inhibitor. Our results present Hibiscus sabdariffa L. and its bioactive compound PCA as potential therapeutic agents in the treatment of HSV-2 infection and the treatment of diseases caused by urease-producing bacteria.
ER  -

HASSAN, STS.; Emil ŠVAJDLENKA a K. BERCHOVA-BIMOVA. Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-Enzymatic Properties against Urease by an ESI-MS Based Assay. \textit{Molecules}. BASEL: Mayer und Muller, 2017, roč.~22, č.~5, 12 s. ISSN~1420-3049. Dostupné z: https://doi.org/10.3390/molecules22050722.

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