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@article{1728040, author = {Zeman, Jiří and Pavloková, Sylvie and Vetchý, David and Pitschmann, Vladimír}, article_number = {1}, keywords = {ethylcellulose; pellets; cholinesterase; detection tube; nerve agent}, language = {eng}, issn = {1210-7816}, journal = {Česká a slovenská farmacie}, title = {Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors}, url = {http://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/dvojite-obalene-pelety-se-semipermeabilnim-obalem-z-ethylcelulosy-urcene-k-detekci-inhibitoru-cholinesteras-122472}, volume = {69}, year = {2020} }
TY - JOUR ID - 1728040 AU - Zeman, Jiří - Pavloková, Sylvie - Vetchý, David - Pitschmann, Vladimír PY - 2020 TI - Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors JF - Česká a slovenská farmacie VL - 69 IS - 1 SP - 24-32 EP - 24-32 PB - Česká lékařská společnost J.E. Purkyně SN - 12107816 KW - ethylcellulose KW - pellets KW - cholinesterase KW - detection tube KW - nerve agent UR - http://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/dvojite-obalene-pelety-se-semipermeabilnim-obalem-z-ethylcelulosy-urcene-k-detekci-inhibitoru-cholinesteras-122472 N2 - Currently, nerve agents are often used in terrorist attacks or assassinations. In such cases, it is necessary to detect them quickly, accurately and easily right in the field. Detection tubes, which are small devices containing pellets with immobilized cholinesterase and detection reagents, meet these conditions. Their detection mechanism is based on a highly sensitive enzymatic Ellman reaction, when in the absence of cholinesterase inhibitors the pellets develop a visible yellow color, whereas in their presence the carriers retain the original color. The rate of reaction, its sensitivity and the distinct color transition are the key points of the research. In this experiment, double-coated pellets were prepared. The first coating contained the butyrylcholinesterase immobilized in hypromellose, while the second coating consisted of ethylcellulose and triethyl citrate. Based on the properties of such carriers, samples containing lactose dispersed in the ethylcellulose coating were also prepared, which was expected to have an effect on increasing the permeability of the coating and hence the detection rate and color intensity. In addition to selected physicochemical properties, carriers were evaluated for enzyme activity, sensitivity and color transition intensity. Samples showing the best properties were subjected to a 24-months stability test at three different temperatures and humidity. ER -
ZEMAN, Jiří, Sylvie PAVLOKOVÁ, David VETCHÝ a Vladimír PITSCHMANN. Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors. \textit{Česká a slovenská farmacie}. Česká lékařská společnost J.E. Purkyně, 2020, roč.~69, č.~1, s.~24-32. ISSN~1210-7816.
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