Enhanced DNA damage response through RAD50 in triple negative
breast cancer resistant and cancer stem-like cells contributes
to chemoresistance

J 2021

Enhanced DNA damage response through RAD50 in triple negative breast cancer resistant and cancer stem-like cells contributes to chemoresistance

ABAD, Etna; Laia CIVIT; David POTĚŠIL; Zbyněk ZDRÁHAL; Alex LYAKHOVICH et al.

Základní údaje

Originální název

Enhanced DNA damage response through RAD50 in triple negative breast cancer resistant and cancer stem-like cells contributes to chemoresistance

Autoři

ABAD, Etna; Laia CIVIT; David POTĚŠIL; Zbyněk ZDRÁHAL a Alex LYAKHOVICH

Vydání

FEBS Journal, Hoboken, Wiley, 2021, 1742-464X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.622

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/21:00118829

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

cancer stem cells; chemoresistance; DNA damage repair; RAD50; triple- negative breast cancer

Štítky

143160, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:49, Ing. Martina Blahová

Anotace

V originále

A growing body of evidence supports the notion that cancer resistance is driven by a small subset of cancer stem cells (CSC), responsible for tumor initiation, growth, and metastasis. Both CSC and chemoresistant cancer cells may share common qualities to activate a series of self-defense mechanisms against chemotherapeutic drugs. Here, we aimed to identify proteins in chemoresistant triple-negative breast cancer (TNBC) cells and corresponding CSC-like spheroid cells that may contribute to their resistance. We have identified several candidate proteins representing the subfamilies of DNA damage response (DDR) system, the ATP-binding cassette, and the 26S proteasome degradation machinery. We have also demonstrated that both cell types exhibit enhanced DDR when compared to corresponding parental counterparts, and identified RAD50 as one of the major contributors in the resistance phenotype. Finally, we have provided evidence that depleting or blocking RAD50 within the Mre11-Rad50-NBS1 (MRN) complex resensitizes CSC and chemoresistant TNBC cells to chemotherapeutic drugs.

Návaznosti

GF19-29701L, projekt VaV

Název: Funkce HDAC1 v T-buněčných lymfomech

Investor: Grantová agentura ČR, Funkce HDAC1 v T-buněčných lymfomech, Partnerská agentura (Rakousko)

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

ABAD, Etna; Laia CIVIT; David POTĚŠIL; Zbyněk ZDRÁHAL a Alex LYAKHOVICH. Enhanced DNA damage response through RAD50 in triple negative breast cancer resistant and cancer stem-like cells contributes to chemoresistance. FEBS Journal. Hoboken: Wiley, 2021, roč. 288, č. 7, s. 2184-2202. ISSN 1742-464X. Dostupné z: https://doi.org/10.1111/febs.15588.

@article{1730032,
   author = {Abad, Etna and Civit, Laia and Potěšil, David and Zdráhal, Zbyněk and Lyakhovich, Alex},
   article_location = {Hoboken},
   article_number = {7},
   doi = {https://doi.org/10.1111/febs.15588},
   keywords = {cancer stem cells; chemoresistance; DNA damage repair; RAD50; triple- negative breast cancer},
   language = {eng},
   issn = {1742-464X},
   journal = {FEBS Journal},
   title = {Enhanced DNA damage response through RAD50 in triple negative breast cancer resistant and cancer stem-like cells contributes to chemoresistance},
   url = {https://doi.org/10.1111/febs.15588},
   volume = {288},
   year = {2021}
}

TY  - JOUR
ID  - 1730032
AU  - Abad, Etna - Civit, Laia - Potěšil, David - Zdráhal, Zbyněk - Lyakhovich, Alex
PY  - 2021
TI  - Enhanced DNA damage response through RAD50 in triple negative breast cancer resistant and cancer stem-like cells contributes to chemoresistance
JF  - FEBS Journal
VL  - 288
IS  - 7
SP  - 2184-2202
EP  - 2184-2202
PB  - Wiley
SN  - 1742464X
KW  - cancer stem cells
KW  - chemoresistance
KW  - DNA damage repair
KW  - RAD50
KW  - triple- negative breast cancer
UR  - https://doi.org/10.1111/febs.15588
L2  - https://doi.org/10.1111/febs.15588
N2  - A growing body of evidence supports the notion that cancer resistance is driven by a small subset of cancer stem cells (CSC), responsible for tumor initiation, growth, and metastasis. Both CSC and chemoresistant cancer cells may share common qualities to activate a series of self-defense mechanisms against chemotherapeutic drugs. Here, we aimed to identify proteins in chemoresistant triple-negative breast cancer (TNBC) cells and corresponding CSC-like spheroid cells that may contribute to their resistance. We have identified several candidate proteins representing the subfamilies of DNA damage response (DDR) system, the ATP-binding cassette, and the 26S proteasome degradation machinery. We have also demonstrated that both cell types exhibit enhanced DDR when compared to corresponding parental counterparts, and identified RAD50 as one of the major contributors in the resistance phenotype. Finally, we have provided evidence that depleting or blocking RAD50 within the Mre11-Rad50-NBS1 (MRN) complex resensitizes CSC and chemoresistant TNBC cells to chemotherapeutic drugs.
ER  -

ABAD, Etna; Laia CIVIT; David POTĚŠIL; Zbyněk ZDRÁHAL a Alex LYAKHOVICH. Enhanced DNA damage response through RAD50 in triple negative breast cancer resistant and cancer stem-like cells contributes to chemoresistance. \textit{FEBS Journal}. Hoboken: Wiley, 2021, roč.~288, č.~7, s.~2184-2202. ISSN~1742-464X. Dostupné z: https://doi.org/10.1111/febs.15588.

Přehled o publikaci