Cytoplasmic maturation in human oocytes: an ultrastructural
study

J 2021

Cytoplasmic maturation in human oocytes: an ultrastructural study

TREBICHALSKÁ, Zuzana; D. KYJOVSKÁ; S. KLOUDOVÁ; P. OTEVŘEL; Aleš HAMPL et al.

Základní údaje

Originální název

Cytoplasmic maturation in human oocytes: an ultrastructural study

Autoři

TREBICHALSKÁ, Zuzana; D. KYJOVSKÁ; S. KLOUDOVÁ; P. OTEVŘEL; Aleš HAMPL a Zuzana HOLUBCOVÁ

Vydání

Biology of Reproduction, Cary, Oxford University Press, 2021, 0006-3363

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10604 Reproductive biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.161

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/21:00118831

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

cytoplasmic maturation; human oocytes

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 2. 2021 08:07, Mgr. Tereza Miškechová

Anotace

V originále

Female fertility relies on successful egg development. Besides chromosome segregation, complex structural and biochemical changes in the cytoplasmic compartment are necessary to confer the female gamete the capacity to undergo normal fertilization and sustain embryonic development. Despite the profound impact on egg quality, morphological bases of cytoplasmic maturation remain largely unknown. Here, we report our findings from the ultrastructural analysis of 69 unfertilized human oocytes from 34 young and healthy egg donors. By comparison of samples fixed at three consecutive developmental stages, we explored how ooplasmic architecture changes during meiotic maturation in vitro. The morphometric image analysis supported observation that the major reorganization of cytoplasm occurs before polar body extrusion. The organelles initially concentrated around prophase nucleus were repositioned toward the periphery and evenly distributed throughout the ooplasm. As maturation progressed, distinct secretory apparatus appeared to transform into cortical granules that clustered underneath the oocyte's surface. The most prominent feature was the gradual formation of heterologous complexes composed of variable elements of endoplasmic reticulum and multiple mitochondria with primitive morphology. Based on the generated image dataset, we proposed a morphological map of cytoplasmic maturation, which may serve as a reference for future comparative studies. In conclusion, this work improves our understanding of human oocyte morphology, cytoplasmic maturation, and intracellular factors defining human egg quality. Although this analysis involved spare oocytes completing development in vitro, it provides essential insight into the enigmatic process by which human egg progenitors prepare for fertilization.

Návaznosti

GJ19-14990Y, projekt VaV

Název: Ultrastrukturní znaky kvality lidských oocytů (Akronym: lidské oocyty)

Investor: Grantová agentura ČR, Ultrastrukturní znaky kvality lidských oocytů.

Citovat

TREBICHALSKÁ, Zuzana; D. KYJOVSKÁ; S. KLOUDOVÁ; P. OTEVŘEL; Aleš HAMPL a Zuzana HOLUBCOVÁ. Cytoplasmic maturation in human oocytes: an ultrastructural study. Biology of Reproduction. Cary: Oxford University Press, 2021, roč. 104, č. 1, s. 106-116. ISSN 0006-3363. Dostupné z: https://doi.org/10.1093/biolre/ioaa174.

@article{1730444,
   author = {Trebichalská, Zuzana and Kyjovská, D. and Kloudová, S. and Otevřel, P. and Hampl, Aleš and Holubcová, Zuzana},
   article_location = {Cary},
   article_number = {1},
   doi = {https://doi.org/10.1093/biolre/ioaa174},
   keywords = {cytoplasmic maturation; human oocytes},
   language = {eng},
   issn = {0006-3363},
   journal = {Biology of Reproduction},
   title = {Cytoplasmic maturation in human oocytes: an ultrastructural study},
   url = {https://academic.oup.com/biolreprod/article/104/1/106/5909494},
   volume = {104},
   year = {2021}
}

TY  - JOUR
ID  - 1730444
AU  - Trebichalská, Zuzana - Kyjovská, D. - Kloudová, S. - Otevřel, P. - Hampl, Aleš - Holubcová, Zuzana
PY  - 2021
TI  - Cytoplasmic maturation in human oocytes: an ultrastructural study
JF  - Biology of Reproduction
VL  - 104
IS  - 1
SP  - 106-116
EP  - 106-116
PB  - Oxford University Press
SN  - 00063363
KW  - cytoplasmic maturation
KW  - human oocytes
UR  - https://academic.oup.com/biolreprod/article/104/1/106/5909494
L2  - https://academic.oup.com/biolreprod/article/104/1/106/5909494
N2  - Female fertility relies on successful egg development. Besides chromosome segregation, complex structural and biochemical changes in the cytoplasmic compartment are necessary to confer the female gamete the capacity to undergo normal fertilization and sustain embryonic development. Despite the profound impact on egg quality, morphological bases of cytoplasmic maturation remain largely unknown. Here, we report our findings from the ultrastructural analysis of 69 unfertilized human oocytes from 34 young and healthy egg donors. By comparison of samples fixed at three consecutive developmental stages, we explored how ooplasmic architecture changes during meiotic maturation in vitro. The morphometric image analysis supported observation that the major reorganization of cytoplasm occurs before polar body extrusion. The organelles initially concentrated around prophase nucleus were repositioned toward the periphery and evenly distributed throughout the ooplasm. As maturation progressed, distinct secretory apparatus appeared to transform into cortical granules that clustered underneath the oocyte's surface. The most prominent feature was the gradual formation of heterologous complexes composed of variable elements of endoplasmic reticulum and multiple mitochondria with primitive morphology. Based on the generated image dataset, we proposed a morphological map of cytoplasmic maturation, which may serve as a reference for future comparative studies. In conclusion, this work improves our understanding of human oocyte morphology, cytoplasmic maturation, and intracellular factors defining human egg quality. Although this analysis involved spare oocytes completing development in vitro, it provides essential insight into the enigmatic process by which human egg progenitors prepare for fertilization.
ER  -

TREBICHALSKÁ, Zuzana; D. KYJOVSKÁ; S. KLOUDOVÁ; P. OTEVŘEL; Aleš HAMPL a Zuzana HOLUBCOVÁ. Cytoplasmic maturation in human oocytes: an ultrastructural study. \textit{Biology of Reproduction}. Cary: Oxford University Press, 2021, roč.~104, č.~1, s.~106-116. ISSN~0006-3363. Dostupné z: https://doi.org/10.1093/biolre/ioaa174.

Přehled o publikaci