Apoptosis related genes expression in primary in vitro culture
of human ovarian granulosa cells.

J 2020

Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells.

KOCHEROVA, I.; K. STEFANSKA; R. BRYL; J. PEREK; W. PIEŃKOWSKI et al.

Základní údaje

Originální název

Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells.

Autoři

KOCHEROVA, I.; K. STEFANSKA; R. BRYL; J. PEREK; W. PIEŃKOWSKI; Jana ŽÁKOVÁ; Igor CRHA; Pavel VENTRUBA; P. MOZDZIAK a Michal JEŠETA

Vydání

Medical Journal of Cell Biology, Walter de Gruyter, 2020, 2544-3577

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/20:00117913

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.2478/acb-2020-0023

EID Scopus

2-s2.0-85100151937

Klíčová slova anglicky

apoptosis-related genes; granulosa cells; primary culture

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 3. 2021 09:45, Mgr. Tereza Miškechová

Anotace

V originále

Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.

Citovat

KOCHEROVA, I.; K. STEFANSKA; R. BRYL; J. PEREK; W. PIEŃKOWSKI; Jana ŽÁKOVÁ; Igor CRHA; Pavel VENTRUBA; P. MOZDZIAK a Michal JEŠETA. Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells. Medical Journal of Cell Biology. Walter de Gruyter, 2020, roč. 8, č. 4, s. 176-182. ISSN 2544-3577. Dostupné z: https://doi.org/10.2478/acb-2020-0023.

@article{1731010,
   author = {Kocherova, I. and Stefanska, K. and Bryl, R. and Perek, J. and Pieńkowski, W. and Žáková, Jana and Crha, Igor and Ventruba, Pavel and Mozdziak, P. and Ješeta, Michal},
   article_number = {4},
   doi = {https://doi.org/10.2478/acb-2020-0023},
   keywords = {apoptosis-related genes; granulosa cells; primary culture},
   language = {eng},
   issn = {2544-3577},
   journal = {Medical Journal of Cell Biology},
   title = {Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells.},
   url = {https://content.sciendo.com/view/journals/acb/8/4/article-p176.xml},
   volume = {8},
   year = {2020}
}

TY  - JOUR
ID  - 1731010
AU  - Kocherova, I. - Stefanska, K. - Bryl, R. - Perek, J. - Pieńkowski, W. - Žáková, Jana - Crha, Igor - Ventruba, Pavel - Mozdziak, P. - Ješeta, Michal
PY  - 2020
TI  - Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells.
JF  - Medical Journal of Cell Biology
VL  - 8
IS  - 4
SP  - 176-182
EP  - 176-182
PB  - Walter de Gruyter
SN  - 25443577
KW  - apoptosis-related genes
KW  - granulosa cells
KW  - primary culture
UR  - https://content.sciendo.com/view/journals/acb/8/4/article-p176.xml
N2  - Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.
ER  -

KOCHEROVA, I.; K. STEFANSKA; R. BRYL; J. PEREK; W. PIE$\backslash$'NKOWSKI; Jana ŽÁKOVÁ; Igor CRHA; Pavel VENTRUBA; P. MOZDZIAK a Michal JEŠETA. Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells. \textit{Medical Journal of Cell Biology}. Walter de Gruyter, 2020, roč.~8, č.~4, s.~176-182. ISSN~2544-3577. Dostupné z: https://doi.org/10.2478/acb-2020-0023.

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