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@inproceedings{1731057,
author = {Outlá, Martina and Spacek, Jan and Renciuk, Daniel and Fojta, Miroslav},
address = {PRAGUE 2},
booktitle = {PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY},
editor = {Nesmerak, K.},
keywords = {circular dichroism spectroscopy; guanine; oxidation; voltammetry},
howpublished = {tištěná verze "print"},
language = {eng},
location = {PRAGUE 2},
isbn = {978-80-7444-068-7},
pages = {232-237},
publisher = {CHARLES UNIV, FAC SCIENCE},
title = {Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode},
year = {2019}
}
TY - JOUR
ID - 1731057
AU - Outlá, Martina - Spacek, Jan - Renciuk, Daniel - Fojta, Miroslav
PY - 2019
TI - Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode
PB - CHARLES UNIV, FAC SCIENCE
CY - PRAGUE 2
SN - 9788074440687
KW - circular dichroism spectroscopy
KW - guanine
KW - oxidation
KW - voltammetry
N2 - The possibility to study DNA quadruplex structures using voltammetric analysis of DNA hexamers was investigated using a pyrolytic graphite electrode in an acetate buffer (pH = 5.0). We have tested the influence of the hexamer sequence and DNA concentration their anodic oxidation. To verify the G quadruplex formation, or lack thereof, circular dichroism spectroscopy was used. Our results suggest that, on the pyrolytic graphite electrode, the guanine oxidation signals are not influenced by formation of G the quadruplex. Instead, the shape of the G oxidation multiple peak can be related to the primary structure of the oligonucleotide.
ER -
OUTLÁ, Martina, Jan SPACEK, Daniel RENCIUK a Miroslav FOJTA. Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode. In Nesmerak, K. \textit{PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY}. PRAGUE 2: CHARLES UNIV, FAC SCIENCE, 2019, s.~232-237. ISBN~978-80-7444-068-7.
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