Phage Amplification Assay for Detection of Mycobacterial
Infection: A Review

J 2021

Phage Amplification Assay for Detection of Mycobacterial Infection: A Review

BEINHAUEROVÁ, Monika a Iva SLANÁ

Základní údaje

Originální název

Phage Amplification Assay for Detection of Mycobacterial Infection: A Review

Autoři

BEINHAUEROVÁ, Monika a Iva SLANÁ

Vydání

Microorganisms, Basel, MDPI, 2021, 2076-2607

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.926

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121115

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Mycobacterium; paratuberculosis; Mycobacterium avium subsp. paratuberculosis; tuberculosis; phage amplification assay; detection; viable cells

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 4. 2021 17:56, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

An important prerequisite for the effective control, timely diagnosis, and successful treatment of mycobacterial infections in both humans and animals is a rapid, specific, and sensitive detection technique. Culture is still considered the gold standard in the detection of viable mycobacteria; however, mycobacteria are extremely fastidious and slow-growing microorganisms, and therefore cultivation requires a very long incubation period to obtain results. Polymerase Chain Reaction (PCR) methods are also frequently used in the diagnosis of mycobacterial infections, providing faster and more accurate results, but are unable to distinguish between a viable and non-viable microorganism, which results in an inability to determine the success of tuberculosis patient treatment or to differentiate between an active and passive infection of animals. One suitable technique that overcomes these shortcomings mentioned is the phage amplification assay (PA). PA specifically detects viable mycobacteria present in a sample within 48 h using a lytic bacteriophage isolated from the environment. Nowadays, an alternative approach to PA, a commercial kit called Actiphage™, is also employed, providing the result within 6–8 h. In this approach, the bacteriophage is used to lyse mycobacterial cells present in the sample, and the released DNA is subsequently detected by PCR. The objective of this review is to summarize information based on the PA used for detection of mycobacteria significant in both human and veterinary medicine from various kinds of matrices.

Citovat

BEINHAUEROVÁ, Monika a Iva SLANÁ. Phage Amplification Assay for Detection of Mycobacterial Infection: A Review. Microorganisms. Basel: MDPI, 2021, roč. 9, č. 2, s. "237", 28 s. ISSN 2076-2607. Dostupné z: https://doi.org/10.3390/microorganisms9020237.

@article{1740176,
   author = {Beinhauerová, Monika and Slaná, Iva},
   article_location = {Basel},
   article_number = {2},
   doi = {https://doi.org/10.3390/microorganisms9020237},
   keywords = {Mycobacterium; paratuberculosis; Mycobacterium avium subsp. paratuberculosis; tuberculosis; phage amplification assay; detection; viable cells},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Phage Amplification Assay for Detection of Mycobacterial Infection: A Review},
   url = {https://www.mdpi.com/2076-2607/9/2/237},
   volume = {9},
   year = {2021}
}

TY  - JOUR
ID  - 1740176
AU  - Beinhauerová, Monika - Slaná, Iva
PY  - 2021
TI  - Phage Amplification Assay for Detection of Mycobacterial Infection: A Review
JF  - Microorganisms
VL  - 9
IS  - 2
SP  - "237"
EP  - "237"
PB  - MDPI
SN  - 20762607
KW  - Mycobacterium
KW  - paratuberculosis
KW  - Mycobacterium avium subsp. paratuberculosis
KW  - tuberculosis
KW  - phage amplification assay
KW  - detection
KW  - viable cells
UR  - https://www.mdpi.com/2076-2607/9/2/237
L2  - https://www.mdpi.com/2076-2607/9/2/237
N2  - An important prerequisite for the effective control, timely diagnosis, and successful treatment of mycobacterial infections in both humans and animals is a rapid, specific, and sensitive detection technique. Culture is still considered the gold standard in the detection of viable mycobacteria; however, mycobacteria are extremely fastidious and slow-growing microorganisms, and therefore cultivation requires a very long incubation period to obtain results. Polymerase Chain Reaction (PCR) methods are also frequently used in the diagnosis of mycobacterial infections, providing faster and more accurate results, but are unable to distinguish between a viable and non-viable microorganism, which results in an inability to determine the success of tuberculosis patient treatment or to differentiate between an active and passive infection of animals. One suitable technique that overcomes these shortcomings mentioned is the phage amplification assay (PA). PA specifically detects viable mycobacteria present in a sample within 48 h using a lytic bacteriophage isolated from the environment. Nowadays, an alternative approach to PA, a commercial kit called Actiphage™, is also employed, providing the result within 6–8 h. In this approach, the bacteriophage is used to lyse mycobacterial cells present in the sample, and the released DNA is subsequently detected by PCR. The objective of this review is to summarize information based on the PA used for detection of mycobacteria significant in both human and veterinary medicine from various kinds of matrices.
ER  -

BEINHAUEROVÁ, Monika a Iva SLANÁ. Phage Amplification Assay for Detection of Mycobacterial Infection: A Review. \textit{Microorganisms}. Basel: MDPI, 2021, roč.~9, č.~2, s.~''237'', 28 s. ISSN~2076-2607. Dostupné z: https://doi.org/10.3390/microorganisms9020237.

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