Evaluating the Influence of a G-Quadruplex Prone Sequence on
the Transactivation Potential by Wild-Type and/or Mutant P53
Family Proteins through a Yeast-Based Functional Assay

J 2021

Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay

MONTI, Paola; Vaclav BRAZDA; Natália BOHÁLOVÁ; Otília PORUBIAKOVÁ; Paola MENICHINI et al.

Základní údaje

Originální název

Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay

Autoři

MONTI, Paola; Vaclav BRAZDA; Natália BOHÁLOVÁ; Otília PORUBIAKOVÁ; Paola MENICHINI; Andrea SPECIALE; Renata BOCCIARDI; Alberto INGA a Gilberto FRONZA

Vydání

Genes, Basel, MDPI, 2021, 2073-4425

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.141

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121141

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

P53 family; yeast; G-quadruplex (G4) prone sequence; wild-type and mutant P53/P63 proteins; transactivation potential

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 4. 2021 17:27, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

P53, P63, and P73 proteins belong to the P53 family of transcription factors, sharing a common gene organization that, from the P1 and P2 promoters, produces two groups of mRNAs encoding proteins with different N-terminal regions; moreover, alternative splicing events at C-terminus further contribute to the generation of multiple isoforms. P53 family proteins can influence a plethora of cellular pathways mainly through the direct binding to specific DNA sequences known as response elements (REs), and the transactivation of the corresponding target genes. However, the transcriptional activation by P53 family members can be regulated at multiple levels, including the DNA topology at responsive promoters. Here, by using a yeast-based functional assay, we evaluated the influence that a G-quadruplex (G4) prone sequence adjacent to the p53 RE derived from the apoptotic PUMA target gene can exert on the transactivation potential of full-length and N-terminal truncated P53 family alpha isoforms (wild-type and mutant). Our results show that the presence of a G4 prone sequence upstream or downstream of the P53 RE leads to significant changes in the relative activity of P53 family proteins, emphasizing the potential role of structural DNA features as modifiers of P53 family functions at target promoter sites.

Citovat

MONTI, Paola; Vaclav BRAZDA; Natália BOHÁLOVÁ; Otília PORUBIAKOVÁ; Paola MENICHINI; Andrea SPECIALE; Renata BOCCIARDI; Alberto INGA a Gilberto FRONZA. Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay. Genes. Basel: MDPI, 2021, roč. 12, č. 2, s. "277", 12 s. ISSN 2073-4425. Dostupné z: https://doi.org/10.3390/genes12020277.

@article{1742738,
   author = {Monti, Paola and Brazda, Vaclav and Bohálová, Natália and Porubiaková, Otília and Menichini, Paola and Speciale, Andrea and Bocciardi, Renata and Inga, Alberto and Fronza, Gilberto},
   article_location = {Basel},
   article_number = {2},
   doi = {https://doi.org/10.3390/genes12020277},
   keywords = {P53 family; yeast; G-quadruplex (G4) prone sequence; wild-type and mutant P53/P63 proteins; transactivation potential},
   language = {eng},
   issn = {2073-4425},
   journal = {Genes},
   title = {Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay},
   url = {https://doi.org/10.3390/genes12020277},
   volume = {12},
   year = {2021}
}

TY  - JOUR
ID  - 1742738
AU  - Monti, Paola - Brazda, Vaclav - Bohálová, Natália - Porubiaková, Otília - Menichini, Paola - Speciale, Andrea - Bocciardi, Renata - Inga, Alberto - Fronza, Gilberto
PY  - 2021
TI  - Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay
JF  - Genes
VL  - 12
IS  - 2
SP  - "277"
EP  - "277"
PB  - MDPI
SN  - 20734425
KW  - P53 family
KW  - yeast
KW  - G-quadruplex (G4) prone sequence
KW  - wild-type and mutant P53/P63 proteins
KW  - transactivation potential
UR  - https://doi.org/10.3390/genes12020277
L2  - https://doi.org/10.3390/genes12020277
N2  - P53, P63, and P73 proteins belong to the P53 family of transcription factors, sharing a common gene organization that, from the P1 and P2 promoters, produces two groups of mRNAs encoding proteins with different N-terminal regions; moreover, alternative splicing events at C-terminus further contribute to the generation of multiple isoforms. P53 family proteins can influence a plethora of cellular pathways mainly through the direct binding to specific DNA sequences known as response elements (REs), and the transactivation of the corresponding target genes. However, the transcriptional activation by P53 family members can be regulated at multiple levels, including the DNA topology at responsive promoters. Here, by using a yeast-based functional assay, we evaluated the influence that a G-quadruplex (G4) prone sequence adjacent to the p53 RE derived from the apoptotic PUMA target gene can exert on the transactivation potential of full-length and N-terminal truncated P53 family alpha isoforms (wild-type and mutant). Our results show that the presence of a G4 prone sequence upstream or downstream of the P53 RE leads to significant changes in the relative activity of P53 family proteins, emphasizing the potential role of structural DNA features as modifiers of P53 family functions at target promoter sites.
ER  -

MONTI, Paola; Vaclav BRAZDA; Natália BOHÁLOVÁ; Otília PORUBIAKOVÁ; Paola MENICHINI; Andrea SPECIALE; Renata BOCCIARDI; Alberto INGA a Gilberto FRONZA. Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay. \textit{Genes}. Basel: MDPI, 2021, roč.~12, č.~2, s.~''277'', 12 s. ISSN~2073-4425. Dostupné z: https://doi.org/10.3390/genes12020277.

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