Black phosphorus enriched matrix-assisted laser desorption
ionization (MALDI) matrices for increased sensitivity of mass
spectrometric detection of biomolecules: Spectrophotometric,
fluorimetric, and mass spectrometric study

k 2021

Black phosphorus enriched matrix-assisted laser desorption ionization (MALDI) matrices for increased sensitivity of mass spectrometric detection of biomolecules: Spectrophotometric, fluorimetric, and mass spectrometric study

MANDAL, Govinda, Lukáš MORÁŇ, Lukáš PEČINKA, Petr VAŇHARA, Josef HAVEL et. al.

Základní údaje

Originální název

Black phosphorus enriched matrix-assisted laser desorption ionization (MALDI) matrices for increased sensitivity of mass spectrometric detection of biomolecules: Spectrophotometric, fluorimetric, and mass spectrometric study

Autoři

MANDAL, Govinda, Lukáš MORÁŇ, Lukáš PEČINKA, Petr VAŇHARA a Josef HAVEL

Vydání

Conference of PhD Students XD 107, Brno, Czech Republic, February 18, 2021. Oral presentation, 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Změněno: 22. 9. 2021 09:38, Govinda Mandal, Ph.D.

Anotace

V originále

We studied the effect of black phosphorus (BP) or phosphorene enriched matrices for ionization of biomolecules in mass spectrometry. It was observed that BP enriched α-cyano-4-hydroxycinnamic acid (CHCA) matrix enhances the intensity of mass spectra significantly in comparison to pure CHCA matrix. In case of amino acids (AAs), the intensities of mass spectra recorded from glycine (Gly), leucine (Leu), phenylalanine (Phe), and tryptophan (Trp) enhanced 26, 50, 30, and 8 times, respectively. The intensities of mass spectra of bradykinin 1-7, angiotensin II, [glu-1]-fibrinopeptide, adrenocorticotropic hormone 1-17 (ACTH 1-17), and ACTH 18-39 standard peptides (Pep) have increased by 35, 28, 8, 4, and 20 times, respectively. The intensities of mass spectra generated from synthetic peptides: A (PRGFSCLLLLTGEIDLP), B (PRGFSCLLLLCGEIDLP), C (PRGFSCLLLLTSEIDLP), and D (MAPRGFSCLLLLTGEIDLPVKRRA) have enhanced 12, 8, 11, and 6 times, respectively. In addition, discrimination of three different cancer cell lines ID8 SCR (mouse ovarian surface epithelial cell line), A2780, and SKOV3 (human ovarian cancer cell lines) has improved from their mass spectra were measured using BP enriched SA matrix. Further, to find the cause of enhanced ionization, interaction of BP fine particles with amino acids or peptides was also investigated using UV-vis spectrophotometry and fluorimetry. It was found that amino acids (Phe and Trp) or peptides containing aromatic rings are interacting with black phosphorus surface (micro or fine BP particles), while Gly or Leu amino acids (containing no aromatic ring) are not. However, this interaction is not connected with their increased ionization using BP enriched CHCA matrix. In the contrary, the ionization of Gly and Leu using BP enriched CHCA matrix is higher than that one observed for Phe and Trp. Therefore, enhanced ionization effect of BP can be mostly explained by surface-assisted laser desorption ionization (SALDI).

Návaznosti

MUNI/A/1421/2019, interní kód MU

Název: Analytické a fyzikálně-chemické metody ve výzkumu geologických, biologických a syntetických materiálů (Akronym: AFCHMAT)

Investor: Masarykova univerzita, Analytické a fyzikálně-chemické metody ve výzkumu geologických, biologických a syntetických materiálů, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/A/1689/2020, interní kód MU

Název: Zdroje pro tkáňové inženýrství 11 (Akronym: TissueEng 11)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 11

NV18-08-00299, projekt VaV

Název: Nové nástroje pro kontrolu kvality pluripotentních kmenových buněk a progenitorů určených pro klinické aplikace

Citovat

MANDAL, Govinda, Lukáš MORÁŇ, Lukáš PEČINKA, Petr VAŇHARA a Josef HAVEL. Black phosphorus enriched matrix-assisted laser desorption ionization (MALDI) matrices for increased sensitivity of mass spectrometric detection of biomolecules: Spectrophotometric, fluorimetric, and mass spectrometric study. In Conference of PhD Students XD 107, Brno, Czech Republic, February 18, 2021. Oral presentation. 2021.

@proceedings{1745438,
   author = {Mandal, Govinda and Moráň, Lukáš and Pečinka, Lukáš and Vaňhara, Petr and Havel, Josef},
   booktitle = {Conference of PhD Students XD 107, Brno, Czech Republic, February 18, 2021. Oral presentation},
   language = {eng},
   title = {Black phosphorus enriched matrix-assisted laser desorption ionization (MALDI) matrices for increased sensitivity of mass spectrometric detection of biomolecules: Spectrophotometric, fluorimetric, and mass spectrometric study},
   year = {2021}
}

TY  - CONF
ID  - 1745438
AU  - Mandal, Govinda - Moráň, Lukáš - Pečinka, Lukáš - Vaňhara, Petr - Havel, Josef
PY  - 2021
TI  - Black phosphorus enriched matrix-assisted laser desorption ionization (MALDI) matrices for increased sensitivity of mass spectrometric detection of biomolecules: Spectrophotometric, fluorimetric, and mass spectrometric study
N2  - We studied the effect of black phosphorus (BP) or phosphorene enriched matrices for ionization of biomolecules in mass spectrometry. It was observed that BP enriched α-cyano-4-hydroxycinnamic acid (CHCA) matrix enhances the intensity of mass spectra significantly in comparison to pure CHCA matrix. In case of amino acids (AAs), the intensities of mass spectra recorded from glycine (Gly), leucine (Leu), phenylalanine (Phe), and tryptophan (Trp) enhanced 26, 50, 30, and 8 times, respectively. The intensities of mass spectra of bradykinin 1-7, angiotensin II, [glu-1]-fibrinopeptide, adrenocorticotropic hormone 1-17 (ACTH 1-17), and ACTH 18-39 standard peptides (Pep) have increased by 35, 28, 8, 4, and 20 times, respectively. The intensities of mass spectra generated from synthetic peptides: A (PRGFSCLLLLTGEIDLP), B (PRGFSCLLLLCGEIDLP), C (PRGFSCLLLLTSEIDLP), and D (MAPRGFSCLLLLTGEIDLPVKRRA) have enhanced 12, 8, 11, and 6 times, respectively. In addition, discrimination of three different cancer cell lines ID8 SCR (mouse ovarian surface epithelial cell line), A2780, and SKOV3 (human ovarian cancer cell lines) has improved from their mass spectra were measured using BP enriched SA matrix. Further, to find the cause of enhanced ionization, interaction of BP fine particles with amino acids or peptides was also investigated using UV-vis spectrophotometry and fluorimetry. It was found that amino acids (Phe and Trp) or peptides containing aromatic rings are interacting with black phosphorus surface (micro or fine BP particles), while Gly or Leu amino acids (containing no aromatic ring) are not. However, this interaction is not connected with their increased ionization using BP enriched CHCA matrix. In the contrary, the ionization of Gly and Leu using BP enriched CHCA matrix is higher than that one observed for Phe and Trp. Therefore, enhanced ionization effect of BP can be mostly explained by surface-assisted laser desorption ionization (SALDI).
ER  -

MANDAL, Govinda, Lukáš MORÁŇ, Lukáš PEČINKA, Petr VAŇHARA a Josef HAVEL. Black phosphorus enriched matrix-assisted laser desorption ionization (MALDI) matrices for increased sensitivity of mass spectrometric detection of biomolecules: Spectrophotometric, fluorimetric, and mass spectrometric study. In \textit{Conference of PhD Students XD 107, Brno, Czech Republic, February 18, 2021. Oral presentation}. 2021.

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