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@article{1746445,
author = {Pastucha, Matěj and Odstrčilíková, Eliška and Hlaváček, Antonín and Brandmeier, Julian and Vykoukal, Vít and Weisová, Julie and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
article_number = {5},
doi = {http://dx.doi.org/10.1109/JSTQE.2021.3049689},
keywords = {American foulbrood; bacterial pathogens; Paenibacillus larvae; photon-upconversion nanoparticle; upconversion-linked immunosorbent assay},
language = {eng},
issn = {1077-260X},
journal = {IEEE Journal of Selected Topics in Quantum Electronics},
title = {Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood},
url = {https://ieeexplore.ieee.org/document/9314922},
volume = {27},
year = {2021}
}
TY - JOUR
ID - 1746445
AU - Pastucha, Matěj - Odstrčilíková, Eliška - Hlaváček, Antonín - Brandmeier, Julian - Vykoukal, Vít - Weisová, Julie - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY - 2021
TI - Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood
JF - IEEE Journal of Selected Topics in Quantum Electronics
VL - 27
IS - 5
SP - 1-11
EP - 1-11
PB - IEEE
SN - 1077260X
KW - American foulbrood
KW - bacterial pathogens
KW - Paenibacillus larvae
KW - photon-upconversion nanoparticle
KW - upconversion-linked immunosorbent assay
UR - https://ieeexplore.ieee.org/document/9314922
N2 - American foulbrood (AFB) caused by the bacterium Paenibacillus larvae is the most destructive disease of the honeybee brood. Therefore, rapid and sensitive detection methods are required to limit spreading of this pathogen, which has a major impact on agriculture and biodiversity. While P. larvae is typically detected by microbial cultivation or polymerase chain reaction, antibody-based detection represents a viable alternative. Here, we prepared an antibody specific for P. larvae and used it for the development of an upconversion-linked immunosorbent assay (ULISA). Photon-upconversion nanoparticles (UCNP) were conjugated to streptavidin via a PEG-linker using copper-catalyzed click chemistry to replace the enzyme label in conventional enzyme-linked immunosorbent assay (ELISA). The ULISA showed low cross-reactivity and provided a limit of detection of 2.9 × 10 3 CFU/mL, representing a 22-fold improvement compared to the ELISA. This level is within the bacterial loads present in honeybee larvae during an AFB infection. The assay was successfully applied to the analysis of spiked samples of bees, larvae, and hive debris.
ER -
PASTUCHA, Matěj, Eliška ODSTRČILÍKOVÁ, Antonín HLAVÁČEK, Julian BRANDMEIER, Vít VYKOUKAL, Julie WEISOVÁ, Hans-Heiner GORRIS, Petr SKLÁDAL a Zdeněk FARKA. Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood. \textit{IEEE Journal of Selected Topics in Quantum Electronics}. IEEE, roč.~27, č.~5, s.~1-11. ISSN~1077-260X. doi:10.1109/JSTQE.2021.3049689. 2021.
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