The Structural Integrity of the Model Lipid Membrane during
Induced Lipid Peroxidation: The Role of Flavonols in the
Inhibition of Lipid Peroxidation

J 2020

The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC et. al.

Základní údaje

Originální název

The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation

Autoři

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC, I. ERCEG, M. KRIECHBAUM, V. STRASSER, Jan PŘIBYL (203 Česká republika, garant, domácí) a S. SEGOTA

Vydání

Antioxidants, Basel, MDPI, 2020, 2076-3921

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.312

Kód RIV

RIV/00216224:14740/20:00118363

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/antiox9050430

UT WoS

000539284200073

Klíčová slova anglicky

bilayer thickness; elasticity; flavonols; fluidity; lipid peroxidation; myricetin; myricitrin; quercetin

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 10. 2024 14:07, Mgr. Adéla Pešková

Anotace

V originále

The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC, I. ERCEG, M. KRIECHBAUM, V. STRASSER, Jan PŘIBYL a S. SEGOTA. The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation. Antioxidants. Basel: MDPI, 2020, roč. 9, č. 5, s. 430-457. ISSN 2076-3921. Dostupné z: https://dx.doi.org/10.3390/antiox9050430.

@article{1751057,
   author = {Sadzak, A. and Mravljak, J. and MaltarandStrmecki, N. and Arsov, Z. and Baranovic, G. and Erceg, I. and Kriechbaum, M. and Strasser, V. and Přibyl, Jan and Segota, S.},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/antiox9050430},
   keywords = {bilayer thickness; elasticity; flavonols; fluidity; lipid peroxidation; myricetin; myricitrin; quercetin},
   language = {eng},
   issn = {2076-3921},
   journal = {Antioxidants},
   title = {The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation},
   url = {https://www.mdpi.com/2076-3921/9/5/430},
   volume = {9},
   year = {2020}
}

TY  - JOUR
ID  - 1751057
AU  - Sadzak, A. - Mravljak, J. - Maltar-Strmecki, N. - Arsov, Z. - Baranovic, G. - Erceg, I. - Kriechbaum, M. - Strasser, V. - Přibyl, Jan - Segota, S.
PY  - 2020
TI  - The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
JF  - Antioxidants
VL  - 9
IS  - 5
SP  - 430
EP  - 430
PB  - MDPI
SN  - 20763921
KW  - bilayer thickness
KW  - elasticity
KW  - flavonols
KW  - fluidity
KW  - lipid peroxidation
KW  - myricetin
KW  - myricitrin
KW  - quercetin
UR  - https://www.mdpi.com/2076-3921/9/5/430
N2  - The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.
ER  -

SADZAK, A., J. MRAVLJAK, N. MALTAR-STRMECKI, Z. ARSOV, G. BARANOVIC, I. ERCEG, M. KRIECHBAUM, V. STRASSER, Jan PŘIBYL a S. SEGOTA. The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation. \textit{Antioxidants}. Basel: MDPI, 2020, roč.~9, č.~5, s.~430-457. ISSN~2076-3921. Dostupné z: https://dx.doi.org/10.3390/antiox9050430.

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