Anti-HA antibody does not detect hyaluronan

J 2021

Anti-HA antibody does not detect hyaluronan

ŠÍNOVÁ, Romana; Petra ŽÁDNÍKOVÁ; Barbora ŠAFRÁNKOVÁ a Kristina NEŠPOROVÁ

Základní údaje

Originální název

Anti-HA antibody does not detect hyaluronan

Autoři

ŠÍNOVÁ, Romana; Petra ŽÁDNÍKOVÁ; Barbora ŠAFRÁNKOVÁ a Kristina NEŠPOROVÁ

Vydání

Glycobiology, Oxford University Press, 2021, 0959-6658

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.954

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121216

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

anti-HA antibody; hyaluronan; hyaluronic acid binding protein; hyaluronidase; IHC; IFC

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 8. 2021 09:17, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

It is generally known that hyaluronic acid (HA) is a biocompatible and biodegradable glycosaminoglycan distributed widely throughout epithelial, connective and neural tissues. HA is one of the chief components of the extracellular matrix. Lack of immunogenicity is one of the biggest advantages of the therapeutic use of HA, but it also prevents the production of specific anti-HA antibodies. Contrary to this, there are still several studies performing HA detection by immunohistochemical or immunohistofluorescent method using an anti-HA antibody. Therefore, this short study discusses whether the anti-HA antibody is specific for HA. To verify the specificity of the HA staining the hyaluronidase treatment of histological samples was performed and the ability of anti-HA antibody and biotinylated HA binding protein (bHABP)-based probe to bind to their targets was evaluated. Additionally, the competitive binding assay with short HA oligosaccharides and subsequent histological staining was performed. Both assays showed that the anti-HA antibody is not sufficiently specific for HA and that the bHABP probe is a reliable method for HA detection in histological samples. The conclusion made by previous investigators based on using HA antibodies should be reevaluated and future use of anti-HA antibody should be avoided.

Citovat

ŠÍNOVÁ, Romana; Petra ŽÁDNÍKOVÁ; Barbora ŠAFRÁNKOVÁ a Kristina NEŠPOROVÁ. Anti-HA antibody does not detect hyaluronan. Glycobiology. Oxford University Press, 2021, roč. 31, č. 5, s. 520-523. ISSN 0959-6658. Dostupné z: https://doi.org/10.1093/glycob/cwaa118.

@article{1751337,
   author = {Šínová, Romana and Žádníková, Petra and Šafránková, Barbora and Nešporová, Kristina},
   article_number = {5},
   doi = {https://doi.org/10.1093/glycob/cwaa118},
   keywords = {anti-HA antibody; hyaluronan; hyaluronic acid binding protein; hyaluronidase; IHC; IFC},
   language = {eng},
   issn = {0959-6658},
   journal = {Glycobiology},
   title = {Anti-HA antibody does not detect hyaluronan},
   url = {https://doi.org/10.1093/glycob/cwaa118},
   volume = {31},
   year = {2021}
}

TY  - JOUR
ID  - 1751337
AU  - Šínová, Romana - Žádníková, Petra - Šafránková, Barbora - Nešporová, Kristina
PY  - 2021
TI  - Anti-HA antibody does not detect hyaluronan
JF  - Glycobiology
VL  - 31
IS  - 5
SP  - 520-523
EP  - 520-523
PB  - Oxford University Press
SN  - 09596658
KW  - anti-HA antibody
KW  - hyaluronan
KW  - hyaluronic acid binding protein
KW  - hyaluronidase
KW  - IHC
KW  - IFC
UR  - https://doi.org/10.1093/glycob/cwaa118
N2  - It is generally known that hyaluronic acid (HA) is a biocompatible and biodegradable glycosaminoglycan distributed widely throughout epithelial, connective and neural tissues. HA is one of the chief components of the extracellular matrix. Lack of immunogenicity is one of the biggest advantages of the therapeutic use of HA, but it also prevents the production of specific anti-HA antibodies. Contrary to this, there are still several studies performing HA detection by immunohistochemical or immunohistofluorescent method using an anti-HA antibody. Therefore, this short study discusses whether the anti-HA antibody is specific for HA. To verify the specificity of the HA staining the hyaluronidase treatment of histological samples was performed and the ability of anti-HA antibody and biotinylated HA binding protein (bHABP)-based probe to bind to their targets was evaluated. Additionally, the competitive binding assay with short HA oligosaccharides and subsequent histological staining was performed. Both assays showed that the anti-HA antibody is not sufficiently specific for HA and that the bHABP probe is a reliable method for HA detection in histological samples. The conclusion made by previous investigators based on using HA antibodies should be reevaluated and future use of anti-HA antibody should be avoided.
ER  -

ŠÍNOVÁ, Romana; Petra ŽÁDNÍKOVÁ; Barbora ŠAFRÁNKOVÁ a Kristina NEŠPOROVÁ. Anti-HA antibody does not detect hyaluronan. \textit{Glycobiology}. Oxford University Press, 2021, roč.~31, č.~5, s.~520-523. ISSN~0959-6658. Dostupné z: https://doi.org/10.1093/glycob/cwaa118.

Přehled o publikaci