Detailed Information on Publication Record
2021
The chromosome-level genome sequence and karyotypic evolution of Megadenia pygmaea (Brassicaceae)
YANG, W.J., L. ZHANG, Terezie MANDÁKOVÁ, L. HUANG, T. LI et. al.Basic information
Original name
The chromosome-level genome sequence and karyotypic evolution of Megadenia pygmaea (Brassicaceae)
Authors
YANG, W.J., L. ZHANG, Terezie MANDÁKOVÁ (203 Czech Republic, belonging to the institution), L. HUANG, T. LI, J.B. JIANG, Y.Z. YANG, Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution), J.Q. LIU and Q.J. HU
Edition
Molecular Ecology Resources, HOBOKEN, WILEY, 2021, 1755-098X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 8.678
RIV identification code
RIV/00216224:14740/21:00121242
Organization unit
Central European Institute of Technology
UT WoS
000594666000001
Keywords in English
Brassicaceae; descending dysploidy; genome assembly; karyotype and chromosome evolution; Qinghai-Tibet Plateau
Tags
Tags
International impact, Reviewed
Změněno: 18/1/2022 13:57, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was assembled for Megadenia pygmaea using Nanopore long reads and high-throughput chromosome conformation capture (Hi-C). The assembled genome is 215.2 Mb and is anchored on six pseudochromosomes. We annotated a total of 25,607 high-confidence protein-coding genes and corroborated the phylogenetic affinity of Megadenia with the Brassicaceae expanded lineage II, containing numerous agricultural crops. We dated the divergence of Megadenia from its closest relatives to 27.04 (19.11-36.60) million years ago. A reconstruction of the chromosomal composition of the species was performed based on the de novo assembled genome and comparative chromosome painting analysis. The karyotype structure of M. pygmaea is very similar to the previously inferred proto-Calepineae karyotype (PCK; n = 7) of the lineage II. However, an end-to-end translocation between two ancestral chromosomes reduced the chromosome number from n = 7 to n = 6 in Megadenia. Our reference genome provides fundamental information for karyotypic evolution and evolutionary study of this genus.
Links
| LQ1601, research and development project |
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