Toward Structure Analysis of a Huge Algal Virus

a 2020

Toward Structure Analysis of a Huge Algal Virus

HOMOLA, Miroslav; Carina Renate BÜTTNER; Tibor FÜZIK a Pavel PLEVKA

Základní údaje

Originální název

Toward Structure Analysis of a Huge Algal Virus

Autoři

HOMOLA, Miroslav; Carina Renate BÜTTNER; Tibor FÜZIK a Pavel PLEVKA

Vydání

CEITEC PhD Conference, 2020

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/20:00118405

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Huge Algal Virus

Štítky

rivok

Změněno: 17. 3. 2021 14:36, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The Emiliania huxleyi virus 86 (EhV-86) is an important agent in terminating regular population explosions of Emiliania huxleyi – the most abundant alga in marine ecosystems. We are attempting to solve the structure of EhV-86 using several cryo-electron microscopy techniques. The unicellular alga Emiliania huxleyi is widely distributed photosynthesizing marine eukaryote, creating regular population explosions called blooms. These algal blooms, visible from space, influence the global climate by absorption of carbon dioxide as E. huxleyi uses this molecule for produc-tion of calcareous plates, covering its surface. The algal bloom collapses usually one week after infection by coccolithoviruses, in most cases by EhV-86. Among the other large double-stranded DNA viruses that infect green algae, EhV-86 differs in possessing a lipid membrane outside of the capsid shell, which it acquires while exiting the algal cell via budding. The membrane and large dimensions of the EhV-86 virus particle make it quite challenging for purification and subsequent 3-dimensional reconstruction. To determine the near-atomic resolution structure of the EhV-86 virion, we use cryo-electron microscopy (cryo-EM). The observed heterogeneity of viral particle morphology pushes reconstruction by so-called single particle analysis, i.e. averaging over many particles – toward tomography, i.e. using only few particles, each imaged at many angles. We also investigate the rep-lication machinery of EhV-86 inside the algal host cell using lamellas – thin cell sections produced by focused ion beam milling. The combination of our results obtained from various cryo-EM approaches will provide a compre-hensive insight into the EhV-86 life cycle and interactions with the Emiliania huxleyi host cell.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

HOMOLA, Miroslav; Carina Renate BÜTTNER; Tibor FÜZIK a Pavel PLEVKA. Toward Structure Analysis of a Huge Algal Virus. In CEITEC PhD Conference. 2020.

@proceedings{1753030,
   author = {Homola, Miroslav and Büttner, Carina Renate and Füzik, Tibor and Plevka, Pavel},
   booktitle = {CEITEC PhD Conference},
   keywords = {Huge Algal Virus},
   language = {eng},
   title = {Toward Structure Analysis of a Huge Algal Virus},
   url = {https://www.ceitec.eu/abstract-book-final-docx-pdf/f36324},
   year = {2020}
}

TY  - CONF
ID  - 1753030
AU  - Homola, Miroslav - Büttner, Carina Renate - Füzik, Tibor - Plevka, Pavel
PY  - 2020
TI  - Toward Structure Analysis of a Huge Algal Virus
KW  - Huge Algal Virus
UR  - https://www.ceitec.eu/abstract-book-final-docx-pdf/f36324
N2  - The Emiliania huxleyi virus 86 (EhV-86) is an important agent in terminating regular population explosions of Emiliania huxleyi – the most abundant alga in marine ecosystems. We are attempting to solve the structure of EhV-86 using several cryo-electron microscopy techniques. The unicellular alga Emiliania huxleyi is widely distributed photosynthesizing marine eukaryote, creating regular population explosions called blooms. These algal blooms, visible from space, influence the global climate by absorption of carbon dioxide as E. huxleyi uses this molecule for produc-tion of calcareous plates, covering its surface. The algal bloom collapses usually one week after infection by coccolithoviruses, in most cases by EhV-86. Among the other large double-stranded DNA viruses that infect green algae, EhV-86 differs in possessing a lipid membrane outside of the capsid shell, which it acquires while exiting the algal cell via budding. The membrane and large dimensions of the EhV-86 virus particle make it quite challenging for purification and subsequent 3-dimensional reconstruction. To determine the near-atomic resolution structure of the EhV-86 virion, we use cryo-electron microscopy (cryo-EM). The observed heterogeneity of viral particle morphology pushes reconstruction by so-called single particle analysis, i.e. averaging over many particles – toward tomography, i.e. using only few particles, each imaged at many angles. We also investigate the rep-lication machinery of EhV-86 inside the algal host cell using lamellas – thin cell sections produced by focused ion beam milling. The combination of our results obtained from various cryo-EM approaches will provide a compre-hensive insight into the EhV-86 life cycle and interactions with the Emiliania huxleyi host cell.
ER  -

HOMOLA, Miroslav; Carina Renate BÜTTNER; Tibor FÜZIK a Pavel PLEVKA. Toward Structure Analysis of a Huge Algal Virus. In \textit{CEITEC PhD Conference}. 2020.

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