A Structural Basis for the Cross-Talk Between Histones and RNA Polymerase II

KASILIAUSKAITE, Aiste, Richard ŠTEFL, Tomáš KLUMPLER and Jiří NOVÁČEK. A Structural Basis for the Cross-Talk Between Histones and RNA Polymerase II. In CEITEC PhD Conference. 2020.

Basic information

• Original name: A Structural Basis for the Cross-Talk Between Histones and RNA Polymerase II
• Authors: KASILIAUSKAITE, Aiste (440 Lithuania, belonging to the institution), Richard ŠTEFL (203 Czech Republic, belonging to the institution), Tomáš KLUMPLER (203 Czech Republic, belonging to the institution) and Jiří NOVÁČEK (203 Czech Republic, belonging to the institution).
• Edition: CEITEC PhD Conference, 2020.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/20:00118407
• Organization unit: Central European Institute of Technology
• Keywords in English: Histones; RNA Polymerase II
• Tags: rivok

Abstract

Transcription of eukaryotic protein-coding genes requires transfer of RNA polymerase II (Pol II) through nucleosomes. Nucleosomes are inherent barriers of transcription, and Pol II stalls at multiple locations within a nucleosome. Nucleosome core particle (NCP) consists of 145–147 base pairs of DNA wrapped around a histone protein octamer. Transcription elongation factors accompany Pol II to facilitate efficient transcription. They enable polymerase progression through NCPs and ensure re-establishment of chromatin after polymerase passage. The mechanisms underlying these processes, however, remain puzzling and poorly understood. Our aim is to present molecular details underlying Spt6 (histone chaperone and transcription factor) binding events. In our study we are revealing this long-standing open question by identifying elements of Spt6 that mediates interactions between Pol II and nucleosome. Cryo-electron microscopy, X-ray and Small Angle X-ray Scattering (SAXS) are used to study the macromolecular complex. Our findings provide a fundamental mechanistic insight into the functional specialization of Spt6 and have implications for the understanding of crosstalk between RNAP II and chromosomes.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR