| DVOŘÁK, Jan, Ondřej CAHA a Dušan HEMZAL. Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers. Plasmonics. New York: Springer, 2021, roč. 16, č. 4, s. 1203-1211. ISSN 1557-1955. Dostupné z: https://dx.doi.org/10.1007/s11468-021-01383-z. |
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@article{1753176,
author = {Dvořák, Jan and Caha, Ondřej and Hemzal, Dušan},
article_location = {New York},
article_number = {4},
doi = {http://dx.doi.org/10.1007/s11468-021-01383-z},
keywords = {Surface plasmon resonance (SPR); Total internal reflection ellipsometry (TIRE); Nanoparticles; Rtt103 160; protein},
language = {eng},
issn = {1557-1955},
journal = {Plasmonics},
title = {Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers},
url = {https://doi.org/10.1007/s11468-021-01383-z},
volume = {16},
year = {2021}
}
TY - JOUR
ID - 1753176
AU - Dvořák, Jan - Caha, Ondřej - Hemzal, Dušan
PY - 2021
TI - Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers
JF - Plasmonics
VL - 16
IS - 4
SP - 1203-1211
EP - 1203-1211
PB - Springer
SN - 15571955
KW - Surface plasmon resonance (SPR)
KW - Total internal reflection ellipsometry (TIRE)
KW - Nanoparticles
KW - Rtt103 160
KW - protein
UR - https://doi.org/10.1007/s11468-021-01383-z
N2 - In this contribution, we quantify the fine-tuning of surface plasmon resonance via nanoparticle coating of gold SPR chips. We target preparation of atomically flat surface with defined charge, needed for scanning probe characterization of biomolecules, with simultaneous optimization of in situ SPR sensitivity during immobilization of these molecules. Using total internal reflection ellipsometry, we show that the goal can be achieved by combination of charge-stabilized silver nanoparticles over underlying gold chip of reduced thickness. The provided formulas for optimal silver to gold thickness relation are valid for arbitrary analyte and the method was experimentally verified on binding of Rtt103 protein.
ER -
DVOŘÁK, Jan, Ondřej CAHA a Dušan HEMZAL. Tuning of SPR for Colocalized Characterization of Biomolecules Using Nanoparticle-Containing Multilayers. \textit{Plasmonics}. New York: Springer, 2021, roč.~16, č.~4, s.~1203-1211. ISSN~1557-1955. Dostupné z: https://dx.doi.org/10.1007/s11468-021-01383-z.
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