Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting
Galactoclusters and Their Interactions with Bacterial Lectin
PA-IL from Pseudomonas aeruginosa

J 2021

Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from Pseudomonas aeruginosa

ILLYÉS, Tünde Zita, Lenka MALINOVSKÁ, Erzsébet RŐTH, Boglárka TÓTH, Bence FARKAS et. al.

Základní údaje

Originální název

Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from Pseudomonas aeruginosa

Autoři

ILLYÉS, Tünde Zita, Lenka MALINOVSKÁ (203 Česká republika, domácí), Erzsébet RŐTH, Boglárka TÓTH, Bence FARKAS, Marek KORSÁK (703 Slovensko, domácí), Michaela WIMMEROVÁ (203 Česká republika, garant, domácí), Katalin E. KÖVÉR a Magdolna CSÁVÁS

Vydání

Molecules, Basel, MDPI, 2021, 1420-3049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.927

Kód RIV

RIV/00216224:14310/21:00118897

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/molecules26030542

UT WoS

000615421600001

Klíčová slova anglicky

selenoglycosides; galactoclusters; Pseudomonas aeruginosa; PA-IL lectin; multivalency

Štítky

CF BIC, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 10. 2024 15:37, Ing. Marie Švancarová

Anotace

V originále

Synthesis of tetravalent thio- and selenogalactopyranoside-containing glycoclusters using azide-alkyne click strategy is presented. Prepared compounds are potential ligands of Pseudomonas aeruginosa lectin PA-IL. P. aeruginosa is an opportunistic human pathogen associated with cystic fibrosis, and PA-IL is one of its virulence factors. The interactions of PA-IL and tetravalent glycoconjugates were investigated using hemagglutination inhibition assay and compared with mono- and divalent galactosides (propargyl 1-thio- and 1-seleno-beta-d-galactopyranoside, digalactosyl diselenide and digalactosyl disulfide). The lectin-carbohydrate interactions were also studied by saturation transfer difference NMR technique. Both thio- and seleno-tetravalent glycoconjugates were able to inhibit PA-IL significantly better than simple d-galactose or their intermediate compounds from the synthesis.

Návaznosti

GA18-18964S, projekt VaV

Název: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Grantová agentura ČR, Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LM2018131, projekt VaV

Název: Česká národní infrastruktura pro biologická data (Akronym: ELIXIR-CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Česká národní infrastruktura pro biologická data

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

ILLYÉS, Tünde Zita, Lenka MALINOVSKÁ, Erzsébet RŐTH, Boglárka TÓTH, Bence FARKAS, Marek KORSÁK, Michaela WIMMEROVÁ, Katalin E. KÖVÉR a Magdolna CSÁVÁS. Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from Pseudomonas aeruginosa. Molecules. Basel: MDPI, 2021, roč. 26, č. 3, s. "542-1"-"542-11", 11 s. ISSN 1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26030542.

@article{1753476,
   author = {Illyés, Tünde Zita and Malinovská, Lenka and Rőth, Erzsébet and Tóth, Boglárka and Farkas, Bence and Korsák, Marek and Wimmerová, Michaela and Kövér, Katalin E. and Csávás, Magdolna},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/molecules26030542},
   keywords = {selenoglycosides; galactoclusters; Pseudomonas aeruginosa; PA-IL lectin; multivalency},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from Pseudomonas aeruginosa},
   url = {https://doi.org/10.3390/molecules26030542},
   volume = {26},
   year = {2021}
}

TY  - JOUR
ID  - 1753476
AU  - Illyés, Tünde Zita - Malinovská, Lenka - Rőth, Erzsébet - Tóth, Boglárka - Farkas, Bence - Korsák, Marek - Wimmerová, Michaela - Kövér, Katalin E. - Csávás, Magdolna
PY  - 2021
TI  - Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from Pseudomonas aeruginosa
JF  - Molecules
VL  - 26
IS  - 3
SP  - "542-1"-"542-11"
EP  - "542-1"-"542-11"
PB  - MDPI
SN  - 14203049
KW  - selenoglycosides
KW  - galactoclusters
KW  - Pseudomonas aeruginosa
KW  - PA-IL lectin
KW  - multivalency
UR  - https://doi.org/10.3390/molecules26030542
N2  - Synthesis of tetravalent thio- and selenogalactopyranoside-containing glycoclusters using azide-alkyne click strategy is presented. Prepared compounds are potential ligands of Pseudomonas aeruginosa lectin PA-IL. P. aeruginosa is an opportunistic human pathogen associated with cystic fibrosis, and PA-IL is one of its virulence factors. The interactions of PA-IL and tetravalent glycoconjugates were investigated using hemagglutination inhibition assay and compared with mono- and divalent galactosides (propargyl 1-thio- and 1-seleno-beta-d-galactopyranoside, digalactosyl diselenide and digalactosyl disulfide). The lectin-carbohydrate interactions were also studied by saturation transfer difference NMR technique. Both thio- and seleno-tetravalent glycoconjugates were able to inhibit PA-IL significantly better than simple d-galactose or their intermediate compounds from the synthesis.
ER  -

ILLYÉS, Tünde Zita, Lenka MALINOVSKÁ, Erzsébet R$\backslash$H OTH, Boglárka TÓTH, Bence FARKAS, Marek KORSÁK, Michaela WIMMEROVÁ, Katalin E. KÖVÉR a Magdolna CSÁVÁS. Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from Pseudomonas aeruginosa. \textit{Molecules}. Basel: MDPI, 2021, roč.~26, č.~3, s.~''542-1''-''542-11'', 11 s. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26030542.

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