ROLE OF PROLINE/GLYCINE KINK IN PORE FORMATION BY ANTIMICROBIAL
PEPTIDES

a 2020

ROLE OF PROLINE/GLYCINE KINK IN PORE FORMATION BY ANTIMICROBIAL PEPTIDES

TÜRKOVÁ, Alžběta; Ivo KABELKA; Lukáš SUKENÍK; Šárka POKORNÁ; Martin Peter HOF et al.

Základní údaje

Originální název

ROLE OF PROLINE/GLYCINE KINK IN PORE FORMATION BY ANTIMICROBIAL PEPTIDES

Autoři

TÜRKOVÁ, Alžběta; Ivo KABELKA ; Lukáš SUKENÍK ; Šárka POKORNÁ; Martin Peter HOF a Robert VÁCHA

Vydání

XXth INTERDISCIPLINARY MEETING OF YOUNG RESEARCHERS AND STUDENTS IN THE FIELD OF CHEMISTRY, BIOCHEMISTRY, MOLECULAR BIOLOGY, AND BIOMATERIALS, 2020

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/20:00118418

Organizační jednotka

Středoevropský technologický institut

ISSN

Klíčová slova anglicky

PROLINE/GLYCINE KINK

Štítky

14312020, 143160, rivok

Změněno: 19. 2. 2023 22:07, prof. RNDr. Robert Vácha, PhD.

Anotace

V originále

Antimicrobial peptides (AMPs) can selectively disrupt bacterial membranes by the formation of leaky pores (see Fig. 1). Their selectivity and potency make them an appealing subject for drug development. Unfortunately, matching the peptide properties with their activity remains elusive. For instance, the role of proline/glycine kink in alfa-helical peptides was reported to both enhance and reduce the antimicrobial activity. In this work, we combined molecular dynamics simulations and fluorescence leakage assays to demonstrate that a helical kink stabilizes toroidal pores but disrupts barrel-stave pores. In addition, the exact position of the proline/glycine kink in the peptide sequence further controls the structure of toroidal pores. The provided molecular-level insight could be utilized for the design and modification of pore-forming antibacterial peptides.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

TÜRKOVÁ, Alžběta; Ivo KABELKA; Lukáš SUKENÍK; Šárka POKORNÁ; Martin Peter HOF a Robert VÁCHA. ROLE OF PROLINE/GLYCINE KINK IN PORE FORMATION BY ANTIMICROBIAL PEPTIDES. In XXth INTERDISCIPLINARY MEETING OF YOUNG RESEARCHERS AND STUDENTS IN THE FIELD OF CHEMISTRY, BIOCHEMISTRY, MOLECULAR BIOLOGY, AND BIOMATERIALS. 2020. ISSN 2336-7202.

@proceedings{1753497,
   author = {Türková, Alžběta and Kabelka, Ivo and Sukeník, Lukáš and Pokorná, Šárka and Hof, Martin Peter and Vácha, Robert},
   booktitle = {XXth INTERDISCIPLINARY MEETING OF YOUNG RESEARCHERS AND STUDENTS IN THE FIELD OF CHEMISTRY, BIOCHEMISTRY, MOLECULAR BIOLOGY, AND BIOMATERIALS},
   keywords = {PROLINE/GLYCINE KINK},
   language = {eng},
   title = {ROLE OF PROLINE/GLYCINE KINK IN PORE FORMATION BY ANTIMICROBIAL PEPTIDES},
   url = {http://www.interdisciplinarymeeting.cz/},
   year = {2020}
}

TY  - CONF
ID  - 1753497
AU  - Türková, Alžběta - Kabelka, Ivo - Sukeník, Lukáš - Pokorná, Šárka - Hof, Martin Peter - Vácha, Robert
PY  - 2020
TI  - ROLE OF PROLINE/GLYCINE KINK IN PORE FORMATION BY ANTIMICROBIAL PEPTIDES
KW  - PROLINE/GLYCINE KINK
UR  - http://www.interdisciplinarymeeting.cz/
N2  - Antimicrobial peptides (AMPs) can selectively disrupt bacterial membranes by the formation of leaky pores (see Fig. 1). Their selectivity and potency make them an appealing subject for drug development. Unfortunately, matching the peptide properties with their activity remains elusive. For instance, the role of proline/glycine kink in alfa-helical peptides was reported to both enhance and reduce the antimicrobial activity. In this work, we combined molecular dynamics simulations and fluorescence leakage assays to demonstrate that a helical kink stabilizes toroidal pores but disrupts barrel-stave pores. In addition, the exact position of the proline/glycine kink in the peptide sequence further controls the structure of toroidal pores. The provided molecular-level insight could be utilized for the design and modification of pore-forming antibacterial peptides.
ER  -

TÜRKOVÁ, Alžběta; Ivo KABELKA; Lukáš SUKENÍK; Šárka POKORNÁ; Martin Peter HOF a Robert VÁCHA. ROLE OF PROLINE/GLYCINE KINK IN PORE FORMATION BY ANTIMICROBIAL PEPTIDES. In \textit{XXth INTERDISCIPLINARY MEETING OF YOUNG RESEARCHERS AND STUDENTS IN THE FIELD OF CHEMISTRY, BIOCHEMISTRY, MOLECULAR BIOLOGY, AND BIOMATERIALS}. 2020. ISSN~2336-7202.

Přehled o publikaci