Methodology for the Implementation of Internal Standard to
Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues

J 2021

Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues

ŠINDELÁŘOVÁ, Anna; Pavel POŘÍZKA; Pavlína MODLITBOVÁ; Lucie VRLÍKOVÁ; Kateřina KISS et al.

Základní údaje

Originální název

Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues

Autoři

ŠINDELÁŘOVÁ, Anna; Pavel POŘÍZKA; Pavlína MODLITBOVÁ; Lucie VRLÍKOVÁ; Kateřina KISS; Milan KAŠKA; David PROCHAZKA; Jakub VRÁBEL; marcela BUCHTOVÁ a Jozef KAISER

Vydání

Sensors, MDPI, 2021, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.847

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121308

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

laser-induced breakdown spectroscopy; elemental mapping; soft tissue ablation; zinc; murine kidneys

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 3. 2021 10:55, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The improving performance of the laser-induced breakdown spectroscopy (LIBS) triggered its utilization in the challenging topic of soft tissue analysis. Alterations of elemental content within soft tissues are commonly assessed and provide further insights in biological research. However, the laser ablation of soft tissues is a complex issue and demands a priori optimization, which is not straightforward in respect to a typical LIBS experiment. Here, we focus on implementing an internal standard into the LIBS elemental analysis of soft tissue samples. We achieve this by extending routine methodology for optimization of soft tissues analysis with a standard spiking method. This step enables a robust optimization procedure of LIBS experimental settings. Considering the implementation of LIBS analysis to the histological routine, we avoid further alterations of the tissue structure. Therefore, we propose a unique methodology of sample preparation, analysis, and subsequent data treatment, which enables the comparison of signal response from heterogenous matrix for different LIBS parameters. Additionally, a brief step-by-step process of optimization to achieve the highest signal-to-noise ratio (SNR) is described. The quality of laser-tissue interaction is investigated on the basis of the zinc signal response, while selected experimental parameters (e.g., defocus, gate delay, laser energy, and ambient atmosphere) are systematically modified.

Citovat

ŠINDELÁŘOVÁ, Anna; Pavel POŘÍZKA; Pavlína MODLITBOVÁ; Lucie VRLÍKOVÁ; Kateřina KISS; Milan KAŠKA; David PROCHAZKA; Jakub VRÁBEL; marcela BUCHTOVÁ a Jozef KAISER. Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues. Sensors. MDPI, 2021, roč. 21, č. 3, s. "900", 13 s. ISSN 1424-8220. Dostupné z: https://doi.org/10.3390/s21030900.

@article{1756196,
   author = {Šindelářová, Anna and Pořízka, Pavel and Modlitbová, Pavlína and Vrlíková, Lucie and Kiss, Kateřina and Kaška, Milan and Prochazka, David and Vrábel, Jakub and Buchtová, marcela and Kaiser, Jozef},
   article_number = {3},
   doi = {https://doi.org/10.3390/s21030900},
   keywords = {laser-induced breakdown spectroscopy; elemental mapping; soft tissue ablation; zinc; murine kidneys},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues},
   url = {https://doi.org/10.3390/s21030900},
   volume = {21},
   year = {2021}
}

TY  - JOUR
ID  - 1756196
AU  - Šindelářová, Anna - Pořízka, Pavel - Modlitbová, Pavlína - Vrlíková, Lucie - Kiss, Kateřina - Kaška, Milan - Prochazka, David - Vrábel, Jakub - Buchtová, marcela - Kaiser, Jozef
PY  - 2021
TI  - Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues
JF  - Sensors
VL  - 21
IS  - 3
SP  - "900"
EP  - "900"
PB  - MDPI
SN  - 14248220
KW  - laser-induced breakdown spectroscopy
KW  - elemental mapping
KW  - soft tissue ablation
KW  - zinc
KW  - murine kidneys
UR  - https://doi.org/10.3390/s21030900
N2  - The improving performance of the laser-induced breakdown spectroscopy (LIBS) triggered its utilization in the challenging topic of soft tissue analysis. Alterations of elemental content within soft tissues are commonly assessed and provide further insights in biological research. However, the laser ablation of soft tissues is a complex issue and demands a priori optimization, which is not straightforward in respect to a typical LIBS experiment. Here, we focus on implementing an internal standard into the LIBS elemental analysis of soft tissue samples. We achieve this by extending routine methodology for optimization of soft tissues analysis with a standard spiking method. This step enables a robust optimization procedure of LIBS experimental settings. Considering the implementation of LIBS analysis to the histological routine, we avoid further alterations of the tissue structure. Therefore, we propose a unique methodology of sample preparation, analysis, and subsequent data treatment, which enables the comparison of signal response from heterogenous matrix for different LIBS parameters. Additionally, a brief step-by-step process of optimization to achieve the highest signal-to-noise ratio (SNR) is described. The quality of laser-tissue interaction is investigated on the basis of the zinc signal response, while selected experimental parameters (e.g., defocus, gate delay, laser energy, and ambient atmosphere) are systematically modified.
ER  -

ŠINDELÁŘOVÁ, Anna; Pavel POŘÍZKA; Pavlína MODLITBOVÁ; Lucie VRLÍKOVÁ; Kateřina KISS; Milan KAŠKA; David PROCHAZKA; Jakub VRÁBEL; marcela BUCHTOVÁ a Jozef KAISER. Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues. \textit{Sensors}. MDPI, 2021, roč.~21, č.~3, s.~''900'', 13 s. ISSN~1424-8220. Dostupné z: https://doi.org/10.3390/s21030900.

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