The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant
Enterobacteriaceae in Tertiary Care Settings

J 2021

The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant Enterobacteriaceae in Tertiary Care Settings

TKADLEC, Jan; Alžběta KALOVÁ; Marie BRAJEROVA; Tereza GELBICOVA; Renata KARPISKOVA et al.

Základní údaje

Originální název

The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant Enterobacteriaceae in Tertiary Care Settings

Autoři

TKADLEC, Jan; Alžběta KALOVÁ; Marie BRAJEROVA; Tereza GELBICOVA; Renata KARPISKOVA; Eva SMELIKOVA; Otakar NYC; Pavel DREVINEK a Marcela KRUTOVA

Vydání

Antibiotics, Basel, MDPI, 2021, 2079-6382

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.222

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121311

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

E. coli; colistin; mcr; Czech Republic; IncX4; silent carriage

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 5. 2021 14:41, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Background: In order to estimate the prevalence of plasmid borne colistin resistance and to characterize in detail the mcr-positive isolates, we carried out a sentinel testing survey on the intestinal carriage of plasmid-mediated colistin-resistant Enterobacteriaceae in hospitalized patients. Methods: Between June 2018 and September 2019, 1922 faecal samples from hospitalised patients were analysed by selective culture in presence of colistin (3.5 mg/L), and in parallel by direct detection of the mcr-1 to mcr-8 genes by qPCR. The mcr-positive isolates were characterised by whole-genome sequencing. Results: The prevalence of the mcr-1 gene was 0.21% (n = 4/1922); the mcr-2 to 8 genes were not detected. The mcr-1 gene was found to be localised in the IncX4 (n = 3) and IncHI2 (n = 1) plasmid type. One Escherichia coli isolate was susceptible to colistin due to the inactivation of the mcr-1 gene through the insertion of the IS2 element; however, the colistin resistance was inducible by culture in low concentrations of colistin. One human mcr-1 positive E. coli isolate was related genetically to the mcr-1 E. coli isolate derived from turkey meat of Czech origin. Conclusions:mcr-mediated colistin resistance currently poses little threat to patients hospitalised in Czech healthcare settings. The presence of the mcr-1 gene in the human population has a possible link to domestically produced, retail meat.

Citovat

TKADLEC, Jan; Alžběta KALOVÁ; Marie BRAJEROVA; Tereza GELBICOVA; Renata KARPISKOVA; Eva SMELIKOVA; Otakar NYC; Pavel DREVINEK a Marcela KRUTOVA. The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant Enterobacteriaceae in Tertiary Care Settings. Antibiotics. Basel: MDPI, 2021, roč. 10, č. 3, s. "258", 12 s. ISSN 2079-6382. Dostupné z: https://doi.org/10.3390/antibiotics10030258.

@article{1756436,
   author = {Tkadlec, Jan and Kalová, Alžběta and Brajerova, Marie and Gelbicova, Tereza and Karpiskova, Renata and Smelikova, Eva and Nyc, Otakar and Drevinek, Pavel and Krutova, Marcela},
   article_location = {Basel},
   article_number = {3},
   doi = {https://doi.org/10.3390/antibiotics10030258},
   keywords = {E. coli; colistin; mcr; Czech Republic; IncX4; silent carriage},
   language = {eng},
   issn = {2079-6382},
   journal = {Antibiotics},
   title = {The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant Enterobacteriaceae in Tertiary Care Settings},
   url = {https://www.mdpi.com/2079-6382/10/3/258/htm},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1756436
AU  - Tkadlec, Jan - Kalová, Alžběta - Brajerova, Marie - Gelbicova, Tereza - Karpiskova, Renata - Smelikova, Eva - Nyc, Otakar - Drevinek, Pavel - Krutova, Marcela
PY  - 2021
TI  - The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant Enterobacteriaceae in Tertiary Care Settings
JF  - Antibiotics
VL  - 10
IS  - 3
SP  - "258"
EP  - "258"
PB  - MDPI
SN  - 20796382
KW  - E. coli
KW  - colistin
KW  - mcr
KW  - Czech Republic
KW  - IncX4
KW  - silent carriage
UR  - https://www.mdpi.com/2079-6382/10/3/258/htm
N2  - Background: In order to estimate the prevalence of plasmid borne colistin resistance and to characterize in detail the mcr-positive isolates, we carried out a sentinel testing survey on the intestinal carriage of plasmid-mediated colistin-resistant Enterobacteriaceae in hospitalized patients. Methods: Between June 2018 and September 2019, 1922 faecal samples from hospitalised patients were analysed by selective culture in presence of colistin (3.5 mg/L), and in parallel by direct detection of the mcr-1 to mcr-8 genes by qPCR. The mcr-positive isolates were characterised by whole-genome sequencing. Results: The prevalence of the mcr-1 gene was 0.21% (n = 4/1922); the mcr-2 to 8 genes were not detected. The mcr-1 gene was found to be localised in the IncX4 (n = 3) and IncHI2 (n = 1) plasmid type. One Escherichia coli isolate was susceptible to colistin due to the inactivation of the mcr-1 gene through the insertion of the IS2 element; however, the colistin resistance was inducible by culture in low concentrations of colistin. One human mcr-1 positive E. coli isolate was related genetically to the mcr-1 E. coli isolate derived from turkey meat of Czech origin. Conclusions:mcr-mediated colistin resistance currently poses little threat to patients hospitalised in Czech healthcare settings. The presence of the mcr-1 gene in the human population has a possible link to domestically produced, retail meat.
ER  -

TKADLEC, Jan; Alžběta KALOVÁ; Marie BRAJEROVA; Tereza GELBICOVA; Renata KARPISKOVA; Eva SMELIKOVA; Otakar NYC; Pavel DREVINEK a Marcela KRUTOVA. The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant Enterobacteriaceae in Tertiary Care Settings. \textit{Antibiotics}. Basel: MDPI, 2021, roč.~10, č.~3, s.~''258'', 12 s. ISSN~2079-6382. Dostupné z: https://doi.org/10.3390/antibiotics10030258.

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