2021
Upconversion immunoassays for environmental control, food safety, and biomedicine
FARKA, Zdeněk, Matthias Jürgen MICKERT, Riikka PELTOMAA, Antonín HLAVÁČEK, Petr SKLÁDAL et. al.Základní údaje
Originální název
Upconversion immunoassays for environmental control, food safety, and biomedicine
Autoři
FARKA, Zdeněk, Matthias Jürgen MICKERT, Riikka PELTOMAA, Antonín HLAVÁČEK, Petr SKLÁDAL a Hans-Heiner GORRIS
Vydání
UPCONline 2021, 2021
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10406 Analytical chemistry
Stát vydavatele
Francie
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Přírodovědecká fakulta
Příznaky
Mezinárodní význam
Změněno: 2. 4. 2021 09:07, doc. Mgr. Zdeněk Farka, Ph.D.
Anotace
V originále
Due to their anti-Stokes emission, photon-upconversion nanoparticles (UCNP) can be detected without optical background interferences. Therefore, the conjugates of UCNPs with appropriate biorecognition molecules are useful labels in immunochemical assays. We have developed upconversion-linked immunosorbent assays (ULISA) for the detection of environmental pollutants, food contaminants, and cancer biomarkers. We have established an indirect and direct ULISA for the pharmaceutical diclofenac (DCF), a common micropollutant in waters. The indirect assay involved a UCNP-labeled secondary antibody, while the tracer in the direct assay consisted of UCNPs enclosed by a carboxylated silica shell and coated with DCF-conjugated bovine γ-globulin. The single-step direct assay provided a limit of detection (LOD) of 20 pg/mL and reduced the analysis time to 70 min. Zearalenone (ZEA) is one of the most frequently encountered mycotoxins in food worldwide. We designed a ZEA-mimicking peptide and used it for the competition with free ZEA in an ULISA. As a label, streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. With an LOD of 20 pg/mL, the ULISA was well-applicable for detecting ZEA at the levels set by the European legislation. Moreover, the unique optical properties enabled imaging of single UCNPs by wide-field epiluminescence microscopy and allowed the development of single-molecule (digital) immunoassays. We have applied the streptavidin-conjugated UCNPs to detect cancer biomarker prostate-specific antigen (PSA). The noise-surpassing digital readout (particle counting) resulted in 20× higher sensitivity than the analog readout (output light intensity) and provided an LOD of 23 fg/mL (800 aM) in 25% human serum.
Návaznosti
| GA21-03156S, projekt VaV |
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| LQ1601, projekt VaV |
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| LTAB19011, projekt VaV |
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