Upconversion immunoassays for environmental control, food
safety, and biomedicine

a 2021

Upconversion immunoassays for environmental control, food safety, and biomedicine

FARKA, Zdeněk, Matthias Jürgen MICKERT, Riikka PELTOMAA, Antonín HLAVÁČEK, Petr SKLÁDAL et. al.

Basic information

Original name

Upconversion immunoassays for environmental control, food safety, and biomedicine

Authors

FARKA, Zdeněk, Matthias Jürgen MICKERT, Riikka PELTOMAA, Antonín HLAVÁČEK, Petr SKLÁDAL and Hans-Heiner GORRIS

Edition

UPCONline 2021, 2021

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Abstract

V originále

Due to their anti-Stokes emission, photon-upconversion nanoparticles (UCNP) can be detected without optical background interferences. Therefore, the conjugates of UCNPs with appropriate biorecognition molecules are useful labels in immunochemical assays. We have developed upconversion-linked immunosorbent assays (ULISA) for the detection of environmental pollutants, food contaminants, and cancer biomarkers. We have established an indirect and direct ULISA for the pharmaceutical diclofenac (DCF), a common micropollutant in waters. The indirect assay involved a UCNP-labeled secondary antibody, while the tracer in the direct assay consisted of UCNPs enclosed by a carboxylated silica shell and coated with DCF-conjugated bovine γ-globulin. The single-step direct assay provided a limit of detection (LOD) of 20 pg/mL and reduced the analysis time to 70 min. Zearalenone (ZEA) is one of the most frequently encountered mycotoxins in food worldwide. We designed a ZEA-mimicking peptide and used it for the competition with free ZEA in an ULISA. As a label, streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. With an LOD of 20 pg/mL, the ULISA was well-applicable for detecting ZEA at the levels set by the European legislation. Moreover, the unique optical properties enabled imaging of single UCNPs by wide-field epiluminescence microscopy and allowed the development of single-molecule (digital) immunoassays. We have applied the streptavidin-conjugated UCNPs to detect cancer biomarker prostate-specific antigen (PSA). The noise-surpassing digital readout (particle counting) resulted in 20× higher sensitivity than the analog readout (output light intensity) and provided an LOD of 23 fg/mL (800 aM) in 25% human serum.

Links

GA21-03156S, research and development project

Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

Citovat

FARKA, Zdeněk, Matthias Jürgen MICKERT, Riikka PELTOMAA, Antonín HLAVÁČEK, Petr SKLÁDAL and Hans-Heiner GORRIS. Upconversion immunoassays for environmental control, food safety, and biomedicine. In UPCONline 2021. 2021.

@proceedings{1757236,
   author = {Farka, Zdeněk and Mickert, Matthias Jürgen and Peltomaa, Riikka and Hlaváček, Antonín and Skládal, Petr and Gorris, HansandHeiner},
   booktitle = {UPCONline 2021},
   language = {eng},
   title = {Upconversion immunoassays for environmental control, food safety, and biomedicine},
   year = {2021}
}

TY  - CONF
ID  - 1757236
AU  - Farka, Zdeněk - Mickert, Matthias Jürgen - Peltomaa, Riikka - Hlaváček, Antonín - Skládal, Petr - Gorris, Hans-Heiner
PY  - 2021
TI  - Upconversion immunoassays for environmental control, food safety, and biomedicine
N2  - Due to their anti-Stokes emission, photon-upconversion nanoparticles (UCNP) can be detected without optical background interferences. Therefore, the conjugates of UCNPs with appropriate biorecognition molecules are useful labels in immunochemical assays. We have developed upconversion-linked immunosorbent assays (ULISA) for the detection of environmental pollutants, food contaminants, and cancer biomarkers. We have established an indirect and direct ULISA for the pharmaceutical diclofenac (DCF), a common micropollutant in waters. The indirect assay involved a UCNP-labeled secondary antibody, while the tracer in the direct assay consisted of UCNPs enclosed by a carboxylated silica shell and coated with DCF-conjugated bovine γ-globulin. The single-step direct assay provided a limit of detection (LOD) of 20 pg/mL and reduced the analysis time to 70 min. Zearalenone (ZEA) is one of the most frequently encountered mycotoxins in food worldwide. We designed a ZEA-mimicking peptide and used it for the competition with free ZEA in an ULISA. As a label, streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. With an LOD of 20 pg/mL, the ULISA was well-applicable for detecting ZEA at the levels set by the European legislation. Moreover, the unique optical properties enabled imaging of single UCNPs by wide-field epiluminescence microscopy and allowed the development of single-molecule (digital) immunoassays. We have applied the streptavidin-conjugated UCNPs to detect cancer biomarker prostate-specific antigen (PSA). The noise-surpassing digital readout (particle counting) resulted in 20× higher sensitivity than the analog readout (output light intensity) and provided an LOD of 23 fg/mL (800 aM) in 25% human serum.
ER  -

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