Rapid detection of antibiotic sensitivity of Staphylococcus
aureus by Raman tweezers

J 2021

Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK et. al.

Základní údaje

Originální název

Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Autoři

BERNATOVA, S. (203 Česká republika, garant), Katarína REBROŠOVÁ (703 Slovensko, domácí), Z. PILAT (203 Česká republika), M. SERY (203 Česká republika), A. GJEVIK (203 Česká republika), O. SAMEK (203 Česká republika), J. JEZEK (203 Česká republika), M. SILER (203 Česká republika), M. KIZOVSKY (203 Česká republika), T. KLEMENTOVA (203 Česká republika), Veronika HOLÁ (203 Česká republika, domácí), Filip RŮŽIČKA (203 Česká republika, domácí) a P. ZEMANEK (203 Česká republika)

Vydání

European Physical Journal Plus, HEIDELBERG, SPRINGER HEIDELBERG, 2021, 2190-5444

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10301 Atomic, molecular and chemical physics

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.758

Kód RIV

RIV/00216224:14110/21:00121360

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1140/epjp/s13360-021-01152-1

UT WoS

000621061800003

Klíčová slova anglicky

antibiotic sensitivity; Staphylococcus aureus; Raman tweezers

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 4. 2021 11:49, Mgr. Tereza Miškechová

Anotace

V originále

Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.

Citovat

BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK, O. SAMEK, J. JEZEK, M. SILER, M. KIZOVSKY, T. KLEMENTOVA, Veronika HOLÁ, Filip RŮŽIČKA a P. ZEMANEK. Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers. European Physical Journal Plus. HEIDELBERG: SPRINGER HEIDELBERG, 2021, roč. 136, č. 2, s. 1-10. ISSN 2190-5444. Dostupné z: https://dx.doi.org/10.1140/epjp/s13360-021-01152-1.

@article{1758059,
   author = {Bernatova, S. and Rebrošová, Katarína and Pilat, Z. and Sery, M. and Gjevik, A. and Samek, O. and Jezek, J. and Siler, M. and Kizovsky, M. and Klementova, T. and Holá, Veronika and Růžička, Filip and Zemanek, P.},
   article_location = {HEIDELBERG},
   article_number = {2},
   doi = {http://dx.doi.org/10.1140/epjp/s13360-021-01152-1},
   keywords = {antibiotic sensitivity; Staphylococcus aureus; Raman tweezers},
   language = {eng},
   issn = {2190-5444},
   journal = {European Physical Journal Plus},
   title = {Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers},
   url = {https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1},
   volume = {136},
   year = {2021}
}

TY  - JOUR
ID  - 1758059
AU  - Bernatova, S. - Rebrošová, Katarína - Pilat, Z. - Sery, M. - Gjevik, A. - Samek, O. - Jezek, J. - Siler, M. - Kizovsky, M. - Klementova, T. - Holá, Veronika - Růžička, Filip - Zemanek, P.
PY  - 2021
TI  - Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers
JF  - European Physical Journal Plus
VL  - 136
IS  - 2
SP  - 1-10
EP  - 1-10
PB  - SPRINGER HEIDELBERG
SN  - 21905444
KW  - antibiotic sensitivity
KW  - Staphylococcus aureus
KW  - Raman tweezers
UR  - https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1
N2  - Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.
ER  -

BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK, O. SAMEK, J. JEZEK, M. SILER, M. KIZOVSKY, T. KLEMENTOVA, Veronika HOLÁ, Filip RŮŽIČKA a P. ZEMANEK. Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers. \textit{European Physical Journal Plus}. HEIDELBERG: SPRINGER HEIDELBERG, 2021, roč.~136, č.~2, s.~1-10. ISSN~2190-5444. Dostupné z: https://dx.doi.org/10.1140/epjp/s13360-021-01152-1.

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