Inkjet-printed electrochemically reduced graphene oxide
microelectrode as a platform for HT-2 mycotoxin immunoenzymatic
biosensing

J 2020

Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing

KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN et. al.

Základní údaje

Originální název

Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing

Autoři

KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA a A. MERKOCI

Vydání

BIOSENSORS & BIOELECTRONICS, OXFORD, ELSEVIER ADVANCED TECHNOLOGY, 2020, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.618

Kód RIV

RIV/00216224:14740/20:00121453

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.bios.2020.112109

UT WoS

000523557300005

Klíčová slova anglicky

2D material; Antibody; Biosensor; Electrochemistry; Graphene oxide

Štítky

CF SAXS, ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 4. 2021 12:23, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 mu m in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectmdes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectmde surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electmactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng . mL(-1) and a linear dynamic range of 6.3 - 100.0 ng . mL(-1) within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA a A. MERKOCI. Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing. BIOSENSORS & BIOELECTRONICS. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2020, roč. 156, MAY, s. 112109-112116. ISSN 0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2020.112109.

@article{1762519,
   author = {Kudr, J. and Zhao, L. and Nguyen, E.P. and Arola, H. and Nevanen, T.K. and Adam, V. and Zitka, O. and Merkoci, A.},
   article_location = {OXFORD},
   article_number = {MAY},
   doi = {http://dx.doi.org/10.1016/j.bios.2020.112109},
   keywords = {2D material; Antibody; Biosensor; Electrochemistry; Graphene oxide},
   language = {eng},
   issn = {0956-5663},
   journal = {BIOSENSORS & BIOELECTRONICS},
   title = {Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing},
   url = {https://www.sciencedirect.com/science/article/pii/S0956566320301068?via%3Dihub},
   volume = {156},
   year = {2020}
}

TY  - JOUR
ID  - 1762519
AU  - Kudr, J. - Zhao, L. - Nguyen, E.P. - Arola, H. - Nevanen, T.K. - Adam, V. - Zitka, O. - Merkoci, A.
PY  - 2020
TI  - Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
JF  - BIOSENSORS & BIOELECTRONICS
VL  - 156
IS  - MAY
SP  - 112109
EP  - 112109
PB  - ELSEVIER ADVANCED TECHNOLOGY
SN  - 09565663
KW  - 2D material
KW  - Antibody
KW  - Biosensor
KW  - Electrochemistry
KW  - Graphene oxide
UR  - https://www.sciencedirect.com/science/article/pii/S0956566320301068?via%3Dihub
N2  - The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 mu m in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectmdes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectmde surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electmactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng . mL(-1) and a linear dynamic range of 6.3 - 100.0 ng . mL(-1) within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.
ER  -

KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA a A. MERKOCI. Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing. \textit{BIOSENSORS \&{}amp; BIOELECTRONICS}. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2020, roč.~156, MAY, s.~112109-112116. ISSN~0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2020.112109.

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