| KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA a A. MERKOCI. Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing. BIOSENSORS & BIOELECTRONICS. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2020, roč. 156, MAY, s. 112109-112116. ISSN 0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2020.112109. |
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@article{1762519,
author = {Kudr, J. and Zhao, L. and Nguyen, E.P. and Arola, H. and Nevanen, T.K. and Adam, V. and Zitka, O. and Merkoci, A.},
article_location = {OXFORD},
article_number = {MAY},
doi = {http://dx.doi.org/10.1016/j.bios.2020.112109},
keywords = {2D material; Antibody; Biosensor; Electrochemistry; Graphene oxide},
language = {eng},
issn = {0956-5663},
journal = {BIOSENSORS & BIOELECTRONICS},
title = {Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing},
url = {https://www.sciencedirect.com/science/article/pii/S0956566320301068?via%3Dihub},
volume = {156},
year = {2020}
}
TY - JOUR
ID - 1762519
AU - Kudr, J. - Zhao, L. - Nguyen, E.P. - Arola, H. - Nevanen, T.K. - Adam, V. - Zitka, O. - Merkoci, A.
PY - 2020
TI - Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
JF - BIOSENSORS & BIOELECTRONICS
VL - 156
IS - MAY
SP - 112109
EP - 112109
PB - ELSEVIER ADVANCED TECHNOLOGY
SN - 09565663
KW - 2D material
KW - Antibody
KW - Biosensor
KW - Electrochemistry
KW - Graphene oxide
UR - https://www.sciencedirect.com/science/article/pii/S0956566320301068?via%3Dihub
N2 - The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 mu m in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectmdes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectmde surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electmactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng . mL(-1) and a linear dynamic range of 6.3 - 100.0 ng . mL(-1) within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.
ER -
KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA a A. MERKOCI. Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing. \textit{BIOSENSORS \&{}amp; BIOELECTRONICS}. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2020, roč.~156, MAY, s.~112109-112116. ISSN~0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2020.112109.
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