The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type
Lamin Depletion Weakens This Interaction

J 2021

The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction

LEGARTOVÁ, Soňa; Paolo FAGHERAZZI; Lenka STIXOVÁ; Aleš KOVAŘÍK; Ivan RAŠKA et al.

Základní údaje

Originální název

The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction

Autoři

LEGARTOVÁ, Soňa; Paolo FAGHERAZZI; Lenka STIXOVÁ; Aleš KOVAŘÍK; Ivan RAŠKA a Eva BÁRTOVÁ

Vydání

Cells, MDPI, 2021, 2073-4409

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.666

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121488

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

splicing; SC-35; PARP inhibitor; RNA pol II

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2021 11:12, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The essential components of splicing are the splicing factors accumulated in nuclear speckles; thus, we studied how DNA damaging agents and A-type lamin depletion affect the properties of these regions, positive on the SC-35 protein. We observed that inhibitor of PARP (poly (ADP-ribose) polymerase), and more pronouncedly inhibitors of RNA polymerases, caused DNA damage and increased the SC-35 protein level. Interestingly, nuclear blebs, induced by PARP inhibitor and observed in A-type lamin-depleted or senescent cells, were positive on both the SC-35 protein and another component of the spliceosome, SRRM2. In the interphase cell nuclei, SC-35 interacted with the phosphorylated form of RNAP II, which was A-type lamin-dependent. In mitotic cells, especially in telophase, the SC-35 protein formed a well-visible ring in the cytoplasmic fraction and colocalized with beta-catenin, associated with the plasma membrane. The antibody against the SRRM2 protein showed that nuclear speckles are already established in the cytoplasm of the late telophase and at the stage of early cytokinesis. In addition, we observed the occurrence of splicing factors in the nuclear blebs and micronuclei, which are also sites of both transcription and splicing. This conclusion supports the fact that splicing proceeds transcriptionally. According to our data, this process is A-type lamin-dependent. Lamin depletion also reduces the interaction between SC-35 and beta-catenin in mitotic cells.

Citovat

LEGARTOVÁ, Soňa; Paolo FAGHERAZZI; Lenka STIXOVÁ; Aleš KOVAŘÍK; Ivan RAŠKA a Eva BÁRTOVÁ. The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction. Cells. MDPI, 2021, roč. 10, č. 2, s. "297", 21 s. ISSN 2073-4409. Dostupné z: https://doi.org/10.3390/cells10020297.

@article{1763998,
   author = {Legartová, Soňa and Fagherazzi, Paolo and Stixová, Lenka and Kovařík, Aleš and Raška, Ivan and Bártová, Eva},
   article_number = {2},
   doi = {https://doi.org/10.3390/cells10020297},
   keywords = {splicing; SC-35; PARP inhibitor; RNA pol II},
   language = {eng},
   issn = {2073-4409},
   journal = {Cells},
   title = {The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction},
   url = {https://www.mdpi.com/2073-4409/10/2/297},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1763998
AU  - Legartová, Soňa - Fagherazzi, Paolo - Stixová, Lenka - Kovařík, Aleš - Raška, Ivan - Bártová, Eva
PY  - 2021
TI  - The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction
JF  - Cells
VL  - 10
IS  - 2
SP  - "297"
EP  - "297"
PB  - MDPI
SN  - 20734409
KW  - splicing
KW  - SC-35
KW  - PARP inhibitor
KW  - RNA pol II
UR  - https://www.mdpi.com/2073-4409/10/2/297
N2  - The essential components of splicing are the splicing factors accumulated in nuclear speckles; thus, we studied how DNA damaging agents and A-type lamin depletion affect the properties of these regions, positive on the SC-35 protein. We observed that inhibitor of PARP (poly (ADP-ribose) polymerase), and more pronouncedly inhibitors of RNA polymerases, caused DNA damage and increased the SC-35 protein level. Interestingly, nuclear blebs, induced by PARP inhibitor and observed in A-type lamin-depleted or senescent cells, were positive on both the SC-35 protein and another component of the spliceosome, SRRM2. In the interphase cell nuclei, SC-35 interacted with the phosphorylated form of RNAP II, which was A-type lamin-dependent. In mitotic cells, especially in telophase, the SC-35 protein formed a well-visible ring in the cytoplasmic fraction and colocalized with beta-catenin, associated with the plasma membrane. The antibody against the SRRM2 protein showed that nuclear speckles are already established in the cytoplasm of the late telophase and at the stage of early cytokinesis. In addition, we observed the occurrence of splicing factors in the nuclear blebs and micronuclei, which are also sites of both transcription and splicing. This conclusion supports the fact that splicing proceeds transcriptionally. According to our data, this process is A-type lamin-dependent. Lamin depletion also reduces the interaction between SC-35 and beta-catenin in mitotic cells.
ER  -

LEGARTOVÁ, Soňa; Paolo FAGHERAZZI; Lenka STIXOVÁ; Aleš KOVAŘÍK; Ivan RAŠKA a Eva BÁRTOVÁ. The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction. \textit{Cells}. MDPI, 2021, roč.~10, č.~2, s.~''297'', 21 s. ISSN~2073-4409. Dostupné z: https://doi.org/10.3390/cells10020297.

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