Další formáty:
BibTeX
LaTeX
RIS
@article{1768639,
author = {Pazourek, Jiří},
article_location = {Weinheim},
article_number = {6-7},
doi = {http://dx.doi.org/10.1002/jssc.200900880},
keywords = {Hydrophilic interaction chromatography; Mutarotation monitoring; Separation of anomers; Separation of pyranose and furanose forms; Single-point calibration},
language = {eng},
issn = {1615-9306},
journal = {Journal of separation science},
title = {Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography},
url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/jssc.200900880},
volume = {33},
year = {2010}
}
TY - JOUR
ID - 1768639
AU - Pazourek, Jiří
PY - 2010
TI - Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography
JF - Journal of separation science
VL - 33
IS - 6-7
SP - 974-981
EP - 974-981
PB - WILEY-V C H VERLAG GMBH
SN - 16159306
KW - Hydrophilic interaction chromatography
KW - Mutarotation monitoring
KW - Separation of anomers
KW - Separation of pyranose and furanose forms
KW - Single-point calibration
UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/jssc.200900880
N2 - Calibration based on the "single-point calibration method", a simple exponential transformation of the response function of an evaporative light scattering detector was improved and applied to analysis of selected saccharides under hydrophilic interaction chromatography mode (a polar phase LiChrospher100 DIOL, mobile phase acetonitrile/water). The improved approach to the calibration procedure yielded a calibration curve with an excellent linearity (quality coefficient <5%). This quantitative evaluation of chromatograms of D-galactose suggested that not only anomers but even pyranose and furanose forms of the anomers could be resolved the resulting calculations of abundance of the anomeric form strongly correlated with data from the literature obtained mostly by NMR studies (analogous results were also obtained for D-arabinose, D-glucose, and D-mannose). Because of the rapid separation (retention time less than 10 min), the observed correlation enabled to monitor anomeric conversion (mutarotation) of monosaccharides.
ER -
PAZOUREK, Jiří. Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography. \textit{Journal of separation science}. Weinheim: WILEY-V C H VERLAG GMBH, 2010, roč.~33, 6-7, s.~974-981. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.200900880.
|
