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@article{1768676,
author = {Pazourek, Jiří},
article_location = {OXFORD},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.carres.2014.03.010},
keywords = {myo-inositol; HILIC; ELSD},
language = {eng},
issn = {0008-6215},
journal = {Carbohydrate Research},
title = {Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0008621514001050},
volume = {391},
year = {2014}
}
TY - JOUR
ID - 1768676
AU - Pazourek, Jiří
PY - 2014
TI - Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography
JF - Carbohydrate Research
VL - 391
IS - 1
SP - 55-60
EP - 55-60
PB - Elsevier Science
SN - 00086215
KW - myo-inositol
KW - HILIC
KW - ELSD
UR - https://www.sciencedirect.com/science/article/abs/pii/S0008621514001050
N2 - A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase could speed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp. (C) 2014 Elsevier Ltd. All rights reserved.
ER -
PAZOUREK, Jiří. Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography. \textit{Carbohydrate Research}. OXFORD: Elsevier Science, 2014, roč.~391, č.~1, s.~55-60. ISSN~0008-6215. Dostupné z: https://dx.doi.org/10.1016/j.carres.2014.03.010.
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