TNFR2 expression is a hallmark of human memory B cells with
suppressive function

J 2021

TNFR2 expression is a hallmark of human memory B cells with suppressive function

TICHA, Olga, Peter SLANINA, Lukas MOOS, Julie ŠTÍCHOVÁ, Marcela VLKOVÁ et. al.

Základní údaje

Originální název

TNFR2 expression is a hallmark of human memory B cells with suppressive function

Autoři

TICHA, Olga (203 Česká republika), Peter SLANINA (703 Slovensko, domácí), Lukas MOOS, Julie ŠTÍCHOVÁ (203 Česká republika, domácí), Marcela VLKOVÁ (203 Česká republika, domácí) a Isabelle BEKEREDJIAN-DING (garant)

Vydání

EUROPEAN JOURNAL OF IMMUNOLOGY, HOBOKEN, WILEY, 2021, 0014-2980

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.688

Kód RIV

RIV/00216224:14110/21:00121608

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/eji.202048988

UT WoS

000626550800001

Klíčová slova anglicky

B cells; Breg; IL‐ 10; TLR9; TNFR2

Štítky

14110114, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 12. 2021 13:44, Mgr. Tereza Miškechová

Anotace

V originále

Tumor Necrosis Factor Receptor 2 (TNFR2) expression is increasingly being linked to tolerogenic immune reactions and cells with suppressor function including a subset of T-regulatory cells. B-regulatory cells play an important role in control of T-cell responses and inflammation. Recently, we described TNFR2 as a marker for IL-10-producing B cells, a hallmark of this cell subset. Here, we demonstrate that proliferation of T cells is reduced in the presence of TNFR2 positive human memory B cells generated with TLR9 ligand, while TNFR2- and TNFR2+CD27- B cells display costimulatory activity. Our data further reveal that IL-10 secretion is characteristic of IgM+ naive and memory B cells but suppressive activity is not restricted to IL-10: (i) the inhibitory effect of TNFR2+ switched memory B cells was comparable to that exerted by TNFR2+ IgM+ memory B cells although IL-10 secretion levels in the cocultures were lower; (ii) supernatants from TNFR2+ memory B cells failed to suppress T-cell proliferation. Based on our findings, we propose that formation of Breg is a specific characteristic of human memory B cells undergoing terminal differentiation. Our data further corroborate that TNFR2 represents a viable marker for identification of memory B cells with regulatory function.

Citovat

TICHA, Olga, Peter SLANINA, Lukas MOOS, Julie ŠTÍCHOVÁ, Marcela VLKOVÁ a Isabelle BEKEREDJIAN-DING. TNFR2 expression is a hallmark of human memory B cells with suppressive function. EUROPEAN JOURNAL OF IMMUNOLOGY. HOBOKEN: WILEY, 2021, roč. 51, č. 5, s. 1195-1205. ISSN 0014-2980. Dostupné z: https://dx.doi.org/10.1002/eji.202048988.

@article{1769698,
   author = {Ticha, Olga and Slanina, Peter and Moos, Lukas and Štíchová, Julie and Vlková, Marcela and BekeredjianandDing, Isabelle},
   article_location = {HOBOKEN},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/eji.202048988},
   keywords = {B cells; Breg; IL‐ 10; TLR9; TNFR2},
   language = {eng},
   issn = {0014-2980},
   journal = {EUROPEAN JOURNAL OF IMMUNOLOGY},
   title = {TNFR2 expression is a hallmark of human memory B cells with suppressive function},
   url = {https://onlinelibrary.wiley.com/journal/15214141},
   volume = {51},
   year = {2021}
}

TY  - JOUR
ID  - 1769698
AU  - Ticha, Olga - Slanina, Peter - Moos, Lukas - Štíchová, Julie - Vlková, Marcela - Bekeredjian-Ding, Isabelle
PY  - 2021
TI  - TNFR2 expression is a hallmark of human memory B cells with suppressive function
JF  - EUROPEAN JOURNAL OF IMMUNOLOGY
VL  - 51
IS  - 5
SP  - 1195-1205
EP  - 1195-1205
PB  - WILEY
SN  - 00142980
KW  - B cells
KW  - Breg
KW  - IL‐ 10
KW  - TLR9
KW  - TNFR2
UR  - https://onlinelibrary.wiley.com/journal/15214141
N2  - Tumor Necrosis Factor Receptor 2 (TNFR2) expression is increasingly being linked to tolerogenic immune reactions and cells with suppressor function including a subset of T-regulatory cells. B-regulatory cells play an important role in control of T-cell responses and inflammation. Recently, we described TNFR2 as a marker for IL-10-producing B cells, a hallmark of this cell subset. Here, we demonstrate that proliferation of T cells is reduced in the presence of TNFR2 positive human memory B cells generated with TLR9 ligand, while TNFR2- and TNFR2+CD27- B cells display costimulatory activity. Our data further reveal that IL-10 secretion is characteristic of IgM+ naive and memory B cells but suppressive activity is not restricted to IL-10: (i) the inhibitory effect of TNFR2+ switched memory B cells was comparable to that exerted by TNFR2+ IgM+ memory B cells although IL-10 secretion levels in the cocultures were lower; (ii) supernatants from TNFR2+ memory B cells failed to suppress T-cell proliferation. Based on our findings, we propose that formation of Breg is a specific characteristic of human memory B cells undergoing terminal differentiation. Our data further corroborate that TNFR2 represents a viable marker for identification of memory B cells with regulatory function.
ER  -

TICHA, Olga, Peter SLANINA, Lukas MOOS, Julie ŠTÍCHOVÁ, Marcela VLKOVÁ a Isabelle BEKEREDJIAN-DING. TNFR2 expression is a hallmark of human memory B cells with suppressive function. \textit{EUROPEAN JOURNAL OF IMMUNOLOGY}. HOBOKEN: WILEY, 2021, roč.~51, č.~5, s.~1195-1205. ISSN~0014-2980. Dostupné z: https://dx.doi.org/10.1002/eji.202048988.

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