Analysis of putative quadruplex-forming sequences in fungal
genomes: novel antifungal targets?

J 2021

Analysis of putative quadruplex-forming sequences in fungal genomes: novel antifungal targets?

WARNER, Emily F.; Natália BOHÁLOVÁ; Václav BRÁZDA; Zoë A. E. WALLER; Stefan BIDULA et al.

Základní údaje

Originální název

Analysis of putative quadruplex-forming sequences in fungal genomes: novel antifungal targets?

Autoři

WARNER, Emily F.; Natália BOHÁLOVÁ; Václav BRÁZDA; Zoë A. E. WALLER a Stefan BIDULA

Vydání

Microbial Genomics, Microbiology Society, 2021, 2057-5858

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.868

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00124935

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Fungi; in-silico; virulence; drug resistance; Aspergillus fumigatus; G-quadruplexes; i-motifs

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 12. 2022 11:42, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Fungal infections cause >1 million deaths annually and the emergence of antifungal resistance has prompted the exploration for novel antifungal targets. Quadruplexes are four-stranded nucleic acid secondary structures, which can regulate processes such as transcription, translation, replication and recombination. They are also found in genes linked to virulence in microbes, and ligands that bind to quadruplexes can eliminate drug-resistant pathogens. Using a computational approach, we quantified putative quadruplex-forming sequences (PQS) in 1359 genomes across the fungal kingdom and explored their presence in genes related to virulence, drug resistance and biological processes associated with pathogenicity in Aspergillus fumigatus. Here we present the largest analysis of PQS in fungi and identify significant heterogeneity of these sequences throughout phyla, genera and species. PQS were genetically conserved in Aspergillus spp. and frequently pathogenic species appeared to contain fewer PQS than their lesser/non-pathogenic counterparts. GO-term analysis identified that PQS-containing genes were involved in processes linked with virulence such as zinc ion binding, the biosynthesis of secondary metabolites and regulation of transcription in A. fumigatus. Although the genome frequency of PQS was lower in A. fumigatus, PQS could be found enriched in genes involved in virulence, and genes upregulated during germination and hypoxia. Moreover, PQS were found in genes involved in drug resistance. Quadruplexes could have important roles within fungal biology and virulence, but their roles require further elucidation.

Citovat

WARNER, Emily F.; Natália BOHÁLOVÁ; Václav BRÁZDA; Zoë A. E. WALLER a Stefan BIDULA. Analysis of putative quadruplex-forming sequences in fungal genomes: novel antifungal targets? Microbial Genomics. Microbiology Society, 2021, roč. 7, č. 5, s. "000570", 18 s. ISSN 2057-5858. Dostupné z: https://doi.org/10.1099/mgen.0.000570.

@article{1772924,
   author = {Warner, Emily F. and Bohálová, Natália and Brázda, Václav and Waller, Zoë A. E. and Bidula, Stefan},
   article_number = {5},
   doi = {https://doi.org/10.1099/mgen.0.000570},
   keywords = {Fungi; in-silico; virulence; drug resistance; Aspergillus fumigatus; G-quadruplexes; i-motifs},
   language = {eng},
   issn = {2057-5858},
   journal = {Microbial Genomics},
   title = {Analysis of putative quadruplex-forming sequences in fungal genomes: novel antifungal targets?},
   url = {https://doi.org/10.1099/mgen.0.000570},
   volume = {7},
   year = {2021}
}

TY  - JOUR
ID  - 1772924
AU  - Warner, Emily F. - Bohálová, Natália - Brázda, Václav - Waller, Zoë A. E. - Bidula, Stefan
PY  - 2021
TI  - Analysis of putative quadruplex-forming sequences in fungal genomes: novel antifungal targets?
JF  - Microbial Genomics
VL  - 7
IS  - 5
SP  - "000570"
EP  - "000570"
PB  - Microbiology Society
SN  - 20575858
KW  - Fungi
KW  - in-silico
KW  - virulence
KW  - drug resistance
KW  - Aspergillus fumigatus
KW  - G-quadruplexes
KW  - i-motifs
UR  - https://doi.org/10.1099/mgen.0.000570
N2  - Fungal infections cause >1 million deaths annually and the emergence of antifungal resistance has prompted the exploration for novel antifungal targets. Quadruplexes are four-stranded nucleic acid secondary structures, which can regulate processes such as transcription, translation, replication and recombination. They are also found in genes linked to virulence in microbes, and ligands that bind to quadruplexes can eliminate drug-resistant pathogens. Using a computational approach, we quantified putative quadruplex-forming sequences (PQS) in 1359 genomes across the fungal kingdom and explored their presence in genes related to virulence, drug resistance and biological processes associated with pathogenicity in Aspergillus fumigatus. Here we present the largest analysis of PQS in fungi and identify significant heterogeneity of these sequences throughout phyla, genera and species. PQS were genetically conserved in Aspergillus spp. and frequently pathogenic species appeared to contain fewer PQS than their lesser/non-pathogenic counterparts. GO-term analysis identified that PQS-containing genes were involved in processes linked with virulence such as zinc ion binding, the biosynthesis of secondary metabolites and regulation of transcription in A. fumigatus. Although the genome frequency of PQS was lower in A. fumigatus, PQS could be found enriched in genes involved in virulence, and genes upregulated during germination and hypoxia. Moreover, PQS were found in genes involved in drug resistance. Quadruplexes could have important roles within fungal biology and virulence, but their roles require further elucidation.
ER  -

WARNER, Emily F.; Natália BOHÁLOVÁ; Václav BRÁZDA; Zo$\backslash$''e A. E. WALLER a Stefan BIDULA. Analysis of putative quadruplex-forming sequences in fungal genomes: novel antifungal targets? \textit{Microbial Genomics}. Microbiology Society, 2021, roč.~7, č.~5, s.~''000570'', 18 s. ISSN~2057-5858. Dostupné z: https://doi.org/10.1099/mgen.0.000570.

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