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@article{1773097,
author = {Škubník, Karel and Sukeník, Lukáš and Buchta, David and Füzik, Tibor and Procházková, Michaela and Moravcová, Jana and Šmerdová, Lenka and Přidal, Antonín and Vácha, Robert and Plevka, Pavel},
article_location = {New York},
article_number = {1},
doi = {http://dx.doi.org/10.1126/sciadv.abd7130},
keywords = {DEFORMED WING VIRUSBEE PARALYSIS VIRUSCRYO-EMMOLECULAR-DYNAMICSFORCE-FIELDX-RAYPOLIOVIRUSRNAMODELTRANSITIONS},
language = {eng},
issn = {2375-2548},
journal = {Science advances},
title = {Capsid opening enables genome release of iflaviruses.},
url = {https://www.science.org/doi/pdf/10.1126/sciadv.abd7130},
volume = {7},
year = {2021}
}
TY - JOUR
ID - 1773097
AU - Škubník, Karel - Sukeník, Lukáš - Buchta, David - Füzik, Tibor - Procházková, Michaela - Moravcová, Jana - Šmerdová, Lenka - Přidal, Antonín - Vácha, Robert - Plevka, Pavel
PY - 2021
TI - Capsid opening enables genome release of iflaviruses.
JF - Science advances
VL - 7
IS - 1
SP - 1-9
EP - 1-9
PB - American Association for the Advancement of Science
SN - 23752548
KW - DEFORMED WING VIRUSBEE PARALYSIS VIRUSCRYO-EMMOLECULAR-DYNAMICSFORCE-FIELDX-RAYPOLIOVIRUSRNAMODELTRANSITIONS
UR - https://www.science.org/doi/pdf/10.1126/sciadv.abd7130
N2 - The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes.
ER -
ŠKUBNÍK, Karel, Lukáš SUKENÍK, David BUCHTA, Tibor FÜZIK, Michaela PROCHÁZKOVÁ, Jana MORAVCOVÁ, Lenka ŠMERDOVÁ, Antonín PŘIDAL, Robert VÁCHA a Pavel PLEVKA. Capsid opening enables genome release of iflaviruses. \textit{Science advances}. New York: American Association for the Advancement of Science, roč.~7, č.~1, s.~1-9. ISSN~2375-2548. doi:10.1126/sciadv.abd7130. 2021.
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