Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis

TUZIL, J., B. FILKOVA, J. MALINA, J. KERESTES a Tomáš DOLEŽAL. Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis. ČESKÁ GYNEKOLOGIE. PRAGUE: NAKLADATELSKE STREDISKO C L S J E PURKYNE, 2021, roč. 86, č. 1, s. 22-29. ISSN 1210-7832. Dostupné z: https://dx.doi.org/10.48095/cccg202122.

Základní údaje

• Originální název: Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis
• Název česky: Kouření u žen s chronickým vaginálním diskomfortem není spojeno se sníženým výskytem Lactobacillus spp. ale podporuje nadměrný růst bakterií Mobiluncus a Gardnerella spp. - sekundární analýza dat z klinické studie zahrnující mikrobiální analýzu
• Autoři: TUZIL, J. (203 Česká republika, garant), B. FILKOVA (203 Česká republika), J. MALINA (203 Česká republika), J. KERESTES (203 Česká republika) a Tomáš DOLEŽAL (203 Česká republika, domácí).
• Vydání: ČESKÁ GYNEKOLOGIE, PRAGUE, NAKLADATELSKE STREDISKO C L S J E PURKYNE, 2021, 1210-7832.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30214 Obstetrics and gynaecology
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Kód RIV: RIV/00216224:14110/21:00121696
• Organizační jednotka: Lékařská fakulta
• Doi: http://dx.doi.org/10.48095/cccg202122
• UT WoS: 000643562000003
• Klíčová slova anglicky: bacterial vaginosis; smoking; Nugent score; vaginal pH; vaginal fl ora; Lactobacillus; Gardnerella; Mobiluncus; human microbiome
• Štítky: 14110516, rivok
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Background: Smoking is considered a risk factor for bacterial vaginosis. It is currently unknown which parameters of the vaginal environment are affected and how smoking triggers the disease. Aim of the study: The primary objective is to estimate the effect size of smoking on vaginal pH and the Nugent score in patients with chronic vulvovaginal discomfort prior to the development of episode of vaginosis. The secondary goal is to investigate the effect of smoking on individual microscopic parameters of the vaginal environment and on subjectively reported symptoms of vaginal discomfort. Methods: Smoking reported by patients was tested as a predictor, using multivariate logistic and ordinal logistic regression analysis on a dataset from the first visit of a randomized trial NCT04171947, which enrolled patients with intermediate vaginal environment. We tested the primary hypothesis (odds ratio (OR) for vaginal pH > 4.5 and Nugent score > 3 in smokers) at the significance level alpha = 5%. For exploratory analyses of the effect of smoking on the parameters of the vaginal environment,. was corrected as per Bonferoni. Results: In a cross-sectional sample of 250 women after adjusting for other risk factors, smoking had an impact on the Nugent score (OR = 3.3 (1.3-8.5), P = 0.011), while pH was not affected (OR = 1.2 (0.5-2.8), P = 0.698). Smoking was associated with the prevalence of clue cells (P < 0.000), Gardnerella spp. (P = 0.001) and Mobiluncus spp. (P = 0.001), while the prevalence of Lactobacillus remained unchanged (P = 0.049). Conclusion: Contrarily to common assumptions, vaginal Lactobacillus is not directly affected by smoking, which rather promotes the growth of bacteria of Gardnerella and Mobiluncus spp. Given that other parameters remained unaffected, it appears that smoking leads to vaginal dysbiosis by creating specific favourable conditions for these two opportunistic pathogens.