Characterisation of Colistin -Resistant Enterobacterales and
Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture
Products

J 2021

Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products

KALOVÁ, Alžběta; Tereza GELBÍČOVÁ; Søren OVERBALLE-PETERSEN; Eva LITRUP; Renáta KARPÍŠKOVÁ et al.

Základní údaje

Originální název

Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products

Autoři

KALOVÁ, Alžběta; Tereza GELBÍČOVÁ; Søren OVERBALLE-PETERSEN; Eva LITRUP a Renáta KARPÍŠKOVÁ

Vydání

Antibiotics, MDPI, 2021, 2079-6382

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.222

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121998

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

colistin; resistance; mcr genes; plasmids

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 8. 2021 09:48, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Aquaculture systems are widely recognised as hotspots for horizontal gene transfer, and the need for screening for bacteria carrying antimicrobial resistance genes in aquaculture systems is becoming more important. In this study, we characterised seventeen bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and A. nosocomialis) resistant to colistin originating from retailed aquaculture products imported from Vietnam to the Czech Republic. The mcr-1.1 gene was found located on plasmid types IncHI2, IncI2, and IncX4, as well as on the rarely described plasmid types IncFIB-FIC and IncFIB(K), phage-like plasmid p0111, and on the chromosome of E. coli. One E. coli strain carried the mcr-3.5 gene on IncFII(pCoo) plasmid in addition to the mcr-1.1 gene located on IncHI2 plasmid. K. pneumoniae was found to carry the mcr-1.1 and mcr-8.2 genes on IncFIA(HI1) plasmid. The mcr-4.3 gene was found on similar untypeable plasmids of A. baumannii and A. nosocomialis strains, pointing to the possible interspecies transfer of plasmids carrying the mcr-4 gene. Our results highlight that some aquaculture products of Asian origin can represent an important source of variable plasmids carrying mcr genes. The results showed an involvement of phages in the incorporation of the mcr-1 gene into plasmids or the chromosome in E. coli strains from aquaculture. The detection of E. coli with the mcr-1 gene in the chromosome points to the risks associated with the stabilisation of the mcr genes in the bacterial chromosome.

Citovat

KALOVÁ, Alžběta; Tereza GELBÍČOVÁ; Søren OVERBALLE-PETERSEN; Eva LITRUP a Renáta KARPÍŠKOVÁ. Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products. Antibiotics. MDPI, 2021, roč. 10, č. 7, s. "838", 16 s. ISSN 2079-6382. Dostupné z: https://doi.org/10.3390/antibiotics10070838.

@article{1784062,
   author = {Kalová, Alžběta and Gelbíčová, Tereza and OverballeandPetersen, Søren and Litrup, Eva and Karpíšková, Renáta},
   article_number = {7},
   doi = {https://doi.org/10.3390/antibiotics10070838},
   keywords = {colistin; resistance; mcr genes; plasmids},
   language = {eng},
   issn = {2079-6382},
   journal = {Antibiotics},
   title = {Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products},
   url = {https://doi.org/10.3390/antibiotics10070838},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1784062
AU  - Kalová, Alžběta - Gelbíčová, Tereza - Overballe-Petersen, Søren - Litrup, Eva - Karpíšková, Renáta
PY  - 2021
TI  - Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products
JF  - Antibiotics
VL  - 10
IS  - 7
SP  - "838"
EP  - "838"
PB  - MDPI
SN  - 20796382
KW  - colistin
KW  - resistance
KW  - mcr genes
KW  - plasmids
UR  - https://doi.org/10.3390/antibiotics10070838
N2  - Aquaculture systems are widely recognised as hotspots for horizontal gene transfer, and the need for screening for bacteria carrying antimicrobial resistance genes in aquaculture systems is becoming more important. In this study, we characterised seventeen bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and A. nosocomialis) resistant to colistin originating from retailed aquaculture products imported from Vietnam to the Czech Republic. The mcr-1.1 gene was found located on plasmid types IncHI2, IncI2, and IncX4, as well as on the rarely described plasmid types IncFIB-FIC and IncFIB(K), phage-like plasmid p0111, and on the chromosome of E. coli. One E. coli strain carried the mcr-3.5 gene on IncFII(pCoo) plasmid in addition to the mcr-1.1 gene located on IncHI2 plasmid. K. pneumoniae was found to carry the mcr-1.1 and mcr-8.2 genes on IncFIA(HI1) plasmid. The mcr-4.3 gene was found on similar untypeable plasmids of A. baumannii and A. nosocomialis strains, pointing to the possible interspecies transfer of plasmids carrying the mcr-4 gene. Our results highlight that some aquaculture products of Asian origin can represent an important source of variable plasmids carrying mcr genes. The results showed an involvement of phages in the incorporation of the mcr-1 gene into plasmids or the chromosome in E. coli strains from aquaculture. The detection of E. coli with the mcr-1 gene in the chromosome points to the risks associated with the stabilisation of the mcr genes in the bacterial chromosome.
ER  -

KALOVÁ, Alžběta; Tereza GELBÍČOVÁ; Søren OVERBALLE-PETERSEN; Eva LITRUP a Renáta KARPÍŠKOVÁ. Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products. \textit{Antibiotics}. MDPI, 2021, roč.~10, č.~7, s.~''838'', 16 s. ISSN~2079-6382. Dostupné z: https://doi.org/10.3390/antibiotics10070838.

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