Simultaneous quantitative profiling of clinically relevant
immune markers in neonatal stool swabs to reveal inflammation

J 2021

Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation

VIDOVÁ, Veronika, Eliška BENEŠOVÁ, Jana KLÁNOVÁ, Vojtěch THON, Zdeněk SPÁČIL et. al.

Základní údaje

Originální název

Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation

Autoři

VIDOVÁ, Veronika (203 Česká republika, domácí), Eliška BENEŠOVÁ (203 Česká republika, domácí), Jana KLÁNOVÁ (203 Česká republika, domácí), Vojtěch THON (203 Česká republika, domácí) a Zdeněk SPÁČIL (203 Česká republika, garant, domácí)

Vydání

Nature Scientific Reports, London, NATURE RESEARCH, 2021, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10700 1.7 Other natural sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.996

Kód RIV

RIV/00216224:14310/21:00119119

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/s41598-021-89384-0

UT WoS

000656946100012

Klíčová slova anglicky

EOSINOPHIL-DERIVED NEUROTOXIN; INTESTINAL INFLAMMATION; FOOD ALLERGY; CESAREAN-SECTION; GRANULE PROTEINS; BOWEL DISEASES; EARLY-LIFE; MECONIUM; GUT; ALPHA-1-ANTITRYPSIN

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 8. 2021 21:38, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

An aberrant immune response developed early in life may trigger inflammatory bowel disease (IBD) and food allergies (e.g., celiac disease). Fecal levels of immune markers categorize an inflammatory response (e.g., food allergy, autoimmune) paralleled with the initial microbial colonization. The immunoaffinity assays are routinely applied to quantify circulating immune protein markers in blood/serum. However, a reliable, multiplex assay to quantify fecal levels of immune proteins is unavailable. We developed mass spectrometry assays to simultaneously quantify fecal calprotectin, myeloperoxidase, eosinophil-derived neurotoxin, eosinophil cationic protein, alpha-1-antitrypsin 1, and adaptive immunity effectors in 134 neonatal stool swabs. We optimized extraction and proteolytic protocol and validated the multiplex assay in terms of linearity of response (>100; typically 0.04 to 14.77 mu g/mg of total protein), coefficient of determination (R-2;>0.99), the limit of detection (LOD; 0.003 to 0.04 mu g/mg of total protein), the limit of quantification (LOQ; 0.009 to 0.122 mu g/mg of total protein) and robustness. The median CV of intra- and interday precision was 9.8% and 14.1%, respectively. We quantified breast milk-derived IGHA2 to differentiate meconium from feces samples and to detect the first food intake. An early life profiling of immune markers reflects disrupted intestinal homeostasis, and it is perhaps suitable for pre-symptomatic interception of IBD and food allergies.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GJ17-24592Y, projekt VaV

Název: Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

Investor: Grantová agentura ČR, Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

Citovat

VIDOVÁ, Veronika, Eliška BENEŠOVÁ, Jana KLÁNOVÁ, Vojtěch THON a Zdeněk SPÁČIL. Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation. Nature Scientific Reports. London: NATURE RESEARCH, 2021, roč. 11, č. 1, s. 1-9. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-021-89384-0.

@article{1785039,
   author = {Vidová, Veronika and Benešová, Eliška and Klánová, Jana and Thon, Vojtěch and Spáčil, Zdeněk},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-021-89384-0},
   keywords = {EOSINOPHIL-DERIVED NEUROTOXIN; INTESTINAL INFLAMMATION; FOOD ALLERGY; CESAREAN-SECTION; GRANULE PROTEINS; BOWEL DISEASES; EARLY-LIFE; MECONIUM; GUT; ALPHA-1-ANTITRYPSIN},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation},
   url = {https://www.nature.com/articles/s41598-021-89384-0},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1785039
AU  - Vidová, Veronika - Benešová, Eliška - Klánová, Jana - Thon, Vojtěch - Spáčil, Zdeněk
PY  - 2021
TI  - Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation
JF  - Nature Scientific Reports
VL  - 11
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - NATURE RESEARCH
SN  - 20452322
KW  - EOSINOPHIL-DERIVED NEUROTOXIN
KW  - INTESTINAL INFLAMMATION
KW  - FOOD ALLERGY
KW  - CESAREAN-SECTION
KW  - GRANULE PROTEINS
KW  - BOWEL DISEASES
KW  - EARLY-LIFE
KW  - MECONIUM
KW  - GUT
KW  - ALPHA-1-ANTITRYPSIN
UR  - https://www.nature.com/articles/s41598-021-89384-0
N2  - An aberrant immune response developed early in life may trigger inflammatory bowel disease (IBD) and food allergies (e.g., celiac disease). Fecal levels of immune markers categorize an inflammatory response (e.g., food allergy, autoimmune) paralleled with the initial microbial colonization. The immunoaffinity assays are routinely applied to quantify circulating immune protein markers in blood/serum. However, a reliable, multiplex assay to quantify fecal levels of immune proteins is unavailable. We developed mass spectrometry assays to simultaneously quantify fecal calprotectin, myeloperoxidase, eosinophil-derived neurotoxin, eosinophil cationic protein, alpha-1-antitrypsin 1, and adaptive immunity effectors in 134 neonatal stool swabs. We optimized extraction and proteolytic protocol and validated the multiplex assay in terms of linearity of response (>100; typically 0.04 to 14.77 mu g/mg of total protein), coefficient of determination (R-2;>0.99), the limit of detection (LOD; 0.003 to 0.04 mu g/mg of total protein), the limit of quantification (LOQ; 0.009 to 0.122 mu g/mg of total protein) and robustness. The median CV of intra- and interday precision was 9.8% and 14.1%, respectively. We quantified breast milk-derived IGHA2 to differentiate meconium from feces samples and to detect the first food intake. An early life profiling of immune markers reflects disrupted intestinal homeostasis, and it is perhaps suitable for pre-symptomatic interception of IBD and food allergies.
ER  -

VIDOVÁ, Veronika, Eliška BENEŠOVÁ, Jana KLÁNOVÁ, Vojtěch THON a Zdeněk SPÁČIL. Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2021, roč.~11, č.~1, s.~1-9. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-021-89384-0.

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