Classification of a Violacein-Producing Psychrophilic Group of
Isolates Associated with Freshwater in Antarctica and
Description of Rugamonas violacea sp. nov.

J 2021

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Roman SOBOTKA, Hans-Jürgen BUSSE, Pavel ŠVEC et. al.

Základní údaje

Originální název

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

Autoři

SEDLÁČEK, Ivo (203 Česká republika, garant, domácí), Pavla HOLOCHOVÁ (203 Česká republika, domácí), Roman SOBOTKA, Hans-Jürgen BUSSE, Pavel ŠVEC (203 Česká republika, domácí), Stanislava KRÁLOVÁ (703 Slovensko, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Jan PILNÝ, Eva STAŇKOVÁ (203 Česká republika, domácí), Vendula KOUBLOVÁ (203 Česká republika, domácí) a Karel SEDLÁŘ

Vydání

Microbiology Spectrum, American Society for Microbiology, 2021, 2165-0497

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.043

Kód RIV

RIV/00216224:14310/21:00122085

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1128/Spectrum.00452-21

UT WoS

000703076500085

Klíčová slova anglicky

psychrophiles; Rugamonas; taxonomy; Antarctica; violacein; species description

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:52, Ing. Martina Blahová

Anotace

V originále

A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, repetitive element sequence-based PCR (rep-PCR), MALDI-TOF MS, fatty acid profile, chemotaxonomy analyses, and extensive biotyping was applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all the isolates constituted a coherent group belonging to the genus Rugamonas. The closest relatives to the representative isolate P5900T were Rugamonas rubra CCM 3730T, Rugamonas rivuli FT103WT, and Rugamonas aquatica FT29WT, exhibiting 99.2%, 99.1%, and 98.6% 16S rRNA pairwise similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values calculated from the whole-genome sequencing data clearly proved that P5900T represents a distinct Rugamonas species. The G+C content of genomic DNAs was 66.1 mol%. The major components in fatty acid profiles were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C 16:0, and C12:0. The cellular quinone content contained exclusively ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The polyamine pattern was composed of putrescine, 2-hydroxputrescine, and spermidine.

Návaznosti

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Roman SOBOTKA, Hans-Jürgen BUSSE, Pavel ŠVEC, Stanislava KRÁLOVÁ, Ondrej ŠEDO, Jan PILNÝ, Eva STAŇKOVÁ, Vendula KOUBLOVÁ a Karel SEDLÁŘ. Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. Microbiology Spectrum. American Society for Microbiology, 2021, roč. 9, č. 1, s. 1-14. ISSN 2165-0497. Dostupné z: https://dx.doi.org/10.1128/Spectrum.00452-21.

@article{1785960,
   author = {Sedláček, Ivo and Holochová, Pavla and Sobotka, Roman and Busse, HansandJürgen and Švec, Pavel and Králová, Stanislava and Šedo, Ondrej and Pilný, Jan and Staňková, Eva and Koublová, Vendula and Sedlář, Karel},
   article_number = {1},
   doi = {http://dx.doi.org/10.1128/Spectrum.00452-21},
   keywords = {psychrophiles; Rugamonas; taxonomy; Antarctica; violacein; species description},
   language = {eng},
   issn = {2165-0497},
   journal = {Microbiology Spectrum},
   title = {Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.},
   url = {https://doi.org/10.1128/Spectrum.00452-21},
   volume = {9},
   year = {2021}
}

TY  - JOUR
ID  - 1785960
AU  - Sedláček, Ivo - Holochová, Pavla - Sobotka, Roman - Busse, Hans-Jürgen - Švec, Pavel - Králová, Stanislava - Šedo, Ondrej - Pilný, Jan - Staňková, Eva - Koublová, Vendula - Sedlář, Karel
PY  - 2021
TI  - Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.
JF  - Microbiology Spectrum
VL  - 9
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - American Society for Microbiology
SN  - 21650497
KW  - psychrophiles
KW  - Rugamonas
KW  - taxonomy
KW  - Antarctica
KW  - violacein
KW  - species description
UR  - https://doi.org/10.1128/Spectrum.00452-21
N2  - A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, repetitive element sequence-based PCR (rep-PCR), MALDI-TOF MS, fatty acid profile, chemotaxonomy analyses, and extensive biotyping was applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all the isolates constituted a coherent group belonging to the genus Rugamonas. The closest relatives to the representative isolate P5900T were Rugamonas rubra CCM 3730T, Rugamonas rivuli FT103WT, and Rugamonas aquatica FT29WT, exhibiting 99.2%, 99.1%, and 98.6% 16S rRNA pairwise similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values calculated from the whole-genome sequencing data clearly proved that P5900T represents a distinct Rugamonas species. The G+C content of genomic DNAs was 66.1 mol%. The major components in fatty acid profiles were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C 16:0, and C12:0. The cellular quinone content contained exclusively ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The polyamine pattern was composed of putrescine, 2-hydroxputrescine, and spermidine.
ER  -

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Roman SOBOTKA, Hans-Jürgen BUSSE, Pavel ŠVEC, Stanislava KRÁLOVÁ, Ondrej ŠEDO, Jan PILNÝ, Eva STAŇKOVÁ, Vendula KOUBLOVÁ a Karel SEDLÁŘ. Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. \textit{Microbiology Spectrum}. American Society for Microbiology, 2021, roč.~9, č.~1, s.~1-14. ISSN~2165-0497. Dostupné z: https://dx.doi.org/10.1128/Spectrum.00452-21.

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