MOL-PCR and xMAP Technology A Multiplex System for Fast
Detection of Food- and Waterborne Viruses

J 2021

MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses

HRDÝ, Jakub; Petra VAŠÍČKOVÁ; Michaela NESVADBOVÁ; Jiří NOVOTNÝ; Tomáš MATI et al.

Základní údaje

Originální název

MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses

Autoři

HRDÝ, Jakub; Petra VAŠÍČKOVÁ ; Michaela NESVADBOVÁ; Jiří NOVOTNÝ; Tomáš MATI a Petr KRÁLÍK

Vydání

The Journal of Molecular Diagnostics, Elsevier, 2021, 1525-1578

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.341

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00122387

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

MOL-PCR; multiplex; complex detection; foodborne infection; waterborne infection; pathogenic viruses

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 11. 2021 10:06, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Viruses are common causes of food- and waterborne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy, or real-time PCR. Because recent technological advancements in detection methods are focused on fast and robust analysis, a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination, was utilized. A new semiquantitative magnetic bead–based multiplex system has been designed for simultaneous detection of several targets in one reaction. The system includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV), and a target for external control of the system. To evaluate the detection system, interlaboratory ring tests were performed in four independent laboratories. Analytical specificity of the tool was tested on a cohort of pathogenic agents and biological samples with quantitative PCR as a reference method. Limit of detection (analytical sensitivity) of 5 × 100 (AdV, HEV, and RVA) and 5 × 101 (HAV and NoV) genome equivalents per reaction was reached. This robust, senstivie, and rapid multiplexing technology may be used to routinely monitor and manage viruses in food and water to prevent food and waterborne diseases.

Citovat

HRDÝ, Jakub; Petra VAŠÍČKOVÁ; Michaela NESVADBOVÁ; Jiří NOVOTNÝ; Tomáš MATI a Petr KRÁLÍK. MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses. The Journal of Molecular Diagnostics. Elsevier, 2021, roč. 23, č. 6, s. 765-776. ISSN 1525-1578. Dostupné z: https://doi.org/10.1016/j.jmoldx.2021.03.005.

@article{1793129,
   author = {Hrdý, Jakub and Vašíčková, Petra and Nesvadbová, Michaela and Novotný, Jiří and Mati, Tomáš and Králík, Petr},
   article_number = {6},
   doi = {https://doi.org/10.1016/j.jmoldx.2021.03.005},
   keywords = {MOL-PCR; multiplex; complex detection; foodborne infection; waterborne infection; pathogenic viruses},
   language = {eng},
   issn = {1525-1578},
   journal = {The Journal of Molecular Diagnostics},
   title = {MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses},
   url = {https://doi.org/10.1016/j.jmoldx.2021.03.005},
   volume = {23},
   year = {2021}
}

TY  - JOUR
ID  - 1793129
AU  - Hrdý, Jakub - Vašíčková, Petra - Nesvadbová, Michaela - Novotný, Jiří - Mati, Tomáš - Králík, Petr
PY  - 2021
TI  - MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses
JF  - The Journal of Molecular Diagnostics
VL  - 23
IS  - 6
SP  - 765-776
EP  - 765-776
PB  - Elsevier
SN  - 15251578
KW  - MOL-PCR
KW  - multiplex
KW  - complex detection
KW  - foodborne infection
KW  - waterborne infection
KW  - pathogenic viruses
UR  - https://doi.org/10.1016/j.jmoldx.2021.03.005
N2  - Viruses are common causes of food- and waterborne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy, or real-time PCR. Because recent technological advancements in detection methods are focused on fast and robust analysis, a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination, was utilized. A new semiquantitative magnetic bead–based multiplex system has been designed for simultaneous detection of several targets in one reaction. The system includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV), and a target for external control of the system. To evaluate the detection system, interlaboratory ring tests were performed in four independent laboratories. Analytical specificity of the tool was tested on a cohort of pathogenic agents and biological samples with quantitative PCR as a reference method. Limit of detection (analytical sensitivity) of 5 × 100 (AdV, HEV, and RVA) and 5 × 101 (HAV and NoV) genome equivalents per reaction was reached. This robust, senstivie, and rapid multiplexing technology may be used to routinely monitor and manage viruses in food and water to prevent food and waterborne diseases.
ER  -

HRDÝ, Jakub; Petra VAŠÍČKOVÁ; Michaela NESVADBOVÁ; Jiří NOVOTNÝ; Tomáš MATI a Petr KRÁLÍK. MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses. \textit{The Journal of Molecular Diagnostics}. Elsevier, 2021, roč.~23, č.~6, s.~765-776. ISSN~1525-1578. Dostupné z: https://doi.org/10.1016/j.jmoldx.2021.03.005.

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