Effect of Particle Size and Surface Chemistry of
Photon-Upconversion Nanoparticles on Analog and Digital
Immunoassays for Cardiac Troponin

J 2021

Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

BRANDMEIER, Julian; Kirsti RAIKO; Zdeněk FARKA; Riikka PELTOMAA; Matthias Jürgen MICKERT et. al.

Základní údaje

Originální název

Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Autoři

BRANDMEIER, Julian; Kirsti RAIKO; Zdeněk FARKA; Riikka PELTOMAA; Matthias Jürgen MICKERT; Antonín HLAVÁČEK; Petr SKLÁDAL; Tero SOUKKA a Hans-Heiner GORRIS

Vydání

Advanced Healthcare Materials, Wiley-VCH, 2021, 2192-2640

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.092

Kód RIV

RIV/00216224:14310/21:00119240

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1002/adhm.202100506

UT WoS

000673454600001

EID Scopus

2-s2.0-85110122799

Klíčová slova anglicky

anti-Stokes emission; cardiac arrest; lanthadine-dopoed nanomaterials; single-molecule immunoassay; troponin; photon-upconversion; upconversion-linked immunosorbent assay

Štítky

14313050, CF CRYO, CF NANO, H23, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 10. 2024 12:27, Ing. Monika Szurmanová, Ph.D.

Anotace

V originále

Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL−1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.

Návaznosti

GA21-03156S, projekt VaV

Název: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Grantová agentura ČR, Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

LTAB19011, projekt VaV

Název: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel, INTER-ACTION

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

BRANDMEIER, Julian; Kirsti RAIKO; Zdeněk FARKA; Riikka PELTOMAA; Matthias Jürgen MICKERT; Antonín HLAVÁČEK; Petr SKLÁDAL; Tero SOUKKA a Hans-Heiner GORRIS. Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin. Advanced Healthcare Materials. Wiley-VCH, 2021, roč. 10, č. 18, s. 1-9. ISSN 2192-2640. Dostupné z: https://doi.org/10.1002/adhm.202100506.

@article{1794867,
   author = {Brandmeier, Julian and Raiko, Kirsti and Farka, Zdeněk and Peltomaa, Riikka and Mickert, Matthias Jürgen and Hlaváček, Antonín and Skládal, Petr and Soukka, Tero and Gorris, HansandHeiner},
   article_number = {18},
   doi = {https://doi.org/10.1002/adhm.202100506},
   keywords = {anti-Stokes emission; cardiac arrest; lanthadine-dopoed nanomaterials; single-molecule immunoassay; troponin; photon-upconversion; upconversion-linked immunosorbent assay},
   language = {eng},
   issn = {2192-2640},
   journal = {Advanced Healthcare Materials},
   title = {Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100506},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1794867
AU  - Brandmeier, Julian - Raiko, Kirsti - Farka, Zdeněk - Peltomaa, Riikka - Mickert, Matthias Jürgen - Hlaváček, Antonín - Skládal, Petr - Soukka, Tero - Gorris, Hans-Heiner
PY  - 2021
TI  - Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin
JF  - Advanced Healthcare Materials
VL  - 10
IS  - 18
SP  - 1-9
EP  - 1-9
PB  - Wiley-VCH
SN  - 21922640
KW  - anti-Stokes emission
KW  - cardiac arrest
KW  - lanthadine-dopoed nanomaterials
KW  - single-molecule immunoassay
KW  - troponin
KW  - photon-upconversion
KW  - upconversion-linked immunosorbent assay
UR  - https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100506
N2  - Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL−1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.
ER  -

BRANDMEIER, Julian; Kirsti RAIKO; Zdeněk FARKA; Riikka PELTOMAA; Matthias Jürgen MICKERT; Antonín HLAVÁČEK; Petr SKLÁDAL; Tero SOUKKA a Hans-Heiner GORRIS. Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin. \textit{Advanced Healthcare Materials}. Wiley-VCH, 2021, roč.~10, č.~18, s.~1-9. ISSN~2192-2640. Dostupné z: https://doi.org/10.1002/adhm.202100506.

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