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@article{1794867,
author = {Brandmeier, Julian and Raiko, Kirsti and Farka, Zdeněk and Peltomaa, Riikka and Mickert, Matthias Jürgen and Hlaváček, Antonín and Skládal, Petr and Soukka, Tero and Gorris, HansandHeiner},
article_number = {18},
doi = {http://dx.doi.org/10.1002/adhm.202100506},
keywords = {anti-Stokes emission; cardiac arrest; lanthadine-dopoed nanomaterials; single-molecule immunoassay; troponin; photon-upconversion; upconversion-linked immunosorbent assay},
language = {eng},
issn = {2192-2640},
journal = {Advanced Healthcare Materials},
title = {Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin},
url = {https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100506},
volume = {10},
year = {2021}
}
TY - JOUR
ID - 1794867
AU - Brandmeier, Julian - Raiko, Kirsti - Farka, Zdeněk - Peltomaa, Riikka - Mickert, Matthias Jürgen - Hlaváček, Antonín - Skládal, Petr - Soukka, Tero - Gorris, Hans-Heiner
PY - 2021
TI - Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin
JF - Advanced Healthcare Materials
VL - 10
IS - 18
SP - 1-9
EP - 1-9
PB - Wiley-VCH
SN - 21922640
KW - anti-Stokes emission
KW - cardiac arrest
KW - lanthadine-dopoed nanomaterials
KW - single-molecule immunoassay
KW - troponin
KW - photon-upconversion
KW - upconversion-linked immunosorbent assay
UR - https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100506
N2 - Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL−1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.
ER -
BRANDMEIER, Julian, Kirsti RAIKO, Zdeněk FARKA, Riikka PELTOMAA, Matthias Jürgen MICKERT, Antonín HLAVÁČEK, Petr SKLÁDAL, Tero SOUKKA a Hans-Heiner GORRIS. Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin. \textit{Advanced Healthcare Materials}. Wiley-VCH, 2021, roč.~10, č.~18, s.~1-9. ISSN~2192-2640. Dostupné z: https://dx.doi.org/10.1002/adhm.202100506.
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