Xeno- and feeder-free derivation of two sex-discordant sibling
lines of human embryonic stem cells

J 2021

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

SOURALOVÁ, Tereza; Zuzana HOLUBCOVÁ; Drahomíra KYJOVSKÁ; Aleš HAMPL; Irena KOUTNÁ et al.

Základní údaje

Originální název

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

Autoři

SOURALOVÁ, Tereza; Zuzana HOLUBCOVÁ; Drahomíra KYJOVSKÁ; Aleš HAMPL a Irena KOUTNÁ

Vydání

Stem Cell Research, Amsterdam, Elsevier, 2021, 1873-5061

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.587

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/21:00120158

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 2. 2022 11:42, Mgr. Tereza Miškechová

Anotace

V originále

Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.

Návaznosti

MUNI/A/1689/2020, interní kód MU

Název: Zdroje pro tkáňové inženýrství 11 (Akronym: TissueEng 11)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 11

NV18-08-00412, projekt VaV

Název: Lidské embryonální kmenové buňky pro klinické aplikace: derivace a charakterizace

Investor: Ministerstvo zdravotnictví ČR, Lidské embryonální kmenové buňky pro klinické aplikace: derivace a charakterizace

Citovat

SOURALOVÁ, Tereza; Zuzana HOLUBCOVÁ; Drahomíra KYJOVSKÁ; Aleš HAMPL a Irena KOUTNÁ. Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells. Stem Cell Research. Amsterdam: Elsevier, 2021, roč. 57, December 2021, s. 1-4. ISSN 1873-5061. Dostupné z: https://doi.org/10.1016/j.scr.2021.102574.

@article{1799997,
   author = {Souralová, Tereza and Holubcová, Zuzana and Kyjovská, Drahomíra and Hampl, Aleš and Koutná, Irena},
   article_location = {Amsterdam},
   article_number = {December 2021},
   doi = {https://doi.org/10.1016/j.scr.2021.102574},
   keywords = {human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines},
   language = {eng},
   issn = {1873-5061},
   journal = {Stem Cell Research},
   title = {Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells},
   url = {https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub},
   volume = {57},
   year = {2021}
}

TY  - JOUR
ID  - 1799997
AU  - Souralová, Tereza - Holubcová, Zuzana - Kyjovská, Drahomíra - Hampl, Aleš - Koutná, Irena
PY  - 2021
TI  - Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells
JF  - Stem Cell Research
VL  - 57
IS  - December 2021
SP  - 1-4
EP  - 1-4
PB  - Elsevier
SN  - 18735061
KW  - human embryonic stem cells
KW  - Xeno- and feeder-free derivation
KW  - two sex-discordant sibling lines
UR  - https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub
N2  - Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
ER  -

SOURALOVÁ, Tereza; Zuzana HOLUBCOVÁ; Drahomíra KYJOVSKÁ; Aleš HAMPL a Irena KOUTNÁ. Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells. \textit{Stem Cell Research}. Amsterdam: Elsevier, 2021, roč.~57, December 2021, s.~1-4. ISSN~1873-5061. Dostupné z: https://doi.org/10.1016/j.scr.2021.102574.

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