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@article{1800681,
author = {Šámalová, Markéta and Moore, Ian},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s12870-021-03241-w},
keywords = {Dexamethasone; Glucocorticoid; Chemically inducible gene expression; GVG; XVE; Promotor; Monocots},
language = {eng},
issn = {1471-2229},
journal = {BMC Plant Biology},
title = {The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa)},
url = {https://doi.org/10.1186/s12870-021-03241-w},
volume = {21},
year = {2021}
}
TY - JOUR
ID - 1800681
AU - Šámalová, Markéta - Moore, Ian
PY - 2021
TI - The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa)
JF - BMC Plant Biology
VL - 21
IS - 1
SP - 461
EP - 461
PB - BioMed Central Ltd.
SN - 14712229
KW - Dexamethasone
KW - Glucocorticoid
KW - Chemically inducible gene expression
KW - GVG
KW - XVE
KW - Promotor
KW - Monocots
UR - https://doi.org/10.1186/s12870-021-03241-w
N2 - Inducible systems for transgene expression activated by a chemical inducer or an inducer of non-plant origin are desirable tools for both basic plant research and biotechnology. Although, the technology has been widely exploited in dicotyledonous model plants such as Arabidopsis, it has not been optimised for use with the monocotyledonous model species, namely rice. We have adapted the dexamethasone-inducible pOp6/LhGR system for rice and the results indicated that it is fast, sensitive and tightly regulated, with high levels of induction that remain stable over several generations. Most importantly, we have shown that the system does not cause negative growth defects in vitro or in soil grown plants. Interestingly in the process of testing, we found that another steroid, triamcinolone acetonide, is a more potent inducer in rice than dexamethasone. We present serious considerations for the construct design to avoid undesirable effects caused by the system in plants, leakiness and possible silencing, as well as simple steps to maximize translation efficiency of a gene of interest. Finally, we compare the performance of the pOp6/LhGR system with other chemically inducible systems tested in rice in terms of the properties of an ideal inducible system.
ER -
ŠÁMALOVÁ, Markéta a Ian MOORE. The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa). \textit{BMC Plant Biology}. London: BioMed Central Ltd., 2021, roč.~21, č.~1, s.~461-475. ISSN~1471-2229. Dostupné z: https://dx.doi.org/10.1186/s12870-021-03241-w.
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