Sanger sequencing of mitochondrial hypervariable region of
ancient samples for DNA authentication and screening before
high-throughput sequencing

J 2021

Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing

ŠENOVSKÁ, Anna; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ; Eva CHOCHOLOVÁ; Dana FIALOVÁ et al.

Základní údaje

Originální název

Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing

Autoři

ŠENOVSKÁ, Anna; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ; Eva CHOCHOLOVÁ ; Dana FIALOVÁ a Jaromír ŠMERDA

Vydání

Journal of Archaeological Science: Reports, Elsevier Ltd. 2021, 2352-409X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00122724

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

ancient DNA; mitochondrial DNA; Sanger sequencing; hypervariable region

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 4. 2023 16:44, Mgr. Eva Chocholová, Ph.D.

Anotace

V originále

Working with ancient DNA is challenging and requires an authentication step. We analyzed eleven badly preserved samples from the Nitra culture with the influence of Únětice culture dated to the 4000 BP Bronze Age. We present estimated mitochondrial haplogroups of four samples based on Sanger sequencing of HVR1 and a part of HVR2. Sanger sequencing has strong limitations in haplogroup prediction, especially when working with ancient DNA. However, Sanger sequencing in this study is featured as a control step to provide us with quality results of mitochondrial DNA and as a screening of present haplogroups. DNA quality estimation is further supported by sample concentration and fragment analysis. The presence of mitochondrial DNA is confirmed by PCR. However, the Sanger sequencing of mitochondrial DNA can confirm its presence in good quality for further sequencing. Therefore, only suitable samples were selected for further high-throughput sequencing of the whole mitogenome.

Návaznosti

MUNI/A/1522/2020, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9 (Akronym: MBG9)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9

Citovat

ŠENOVSKÁ, Anna; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ; Eva CHOCHOLOVÁ; Dana FIALOVÁ a Jaromír ŠMERDA. Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing. Journal of Archaeological Science: Reports. Elsevier Ltd., 2021, roč. 40, December, s. 1-5. ISSN 2352-409X. Dostupné z: https://doi.org/10.1016/j.jasrep.2021.103216.

@article{1800817,
   author = {Šenovská, Anna and Drozdová, Eva and Brzobohatá, Kristýna and Chocholová, Eva and Fialová, Dana and Šmerda, Jaromír},
   article_number = {December},
   doi = {https://doi.org/10.1016/j.jasrep.2021.103216},
   keywords = {ancient DNA; mitochondrial DNA; Sanger sequencing; hypervariable region},
   language = {eng},
   issn = {2352-409X},
   journal = {Journal of Archaeological Science: Reports},
   title = {Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing},
   url = {https://doi.org/10.1016/j.jasrep.2021.103216},
   volume = {40},
   year = {2021}
}

TY  - JOUR
ID  - 1800817
AU  - Šenovská, Anna - Drozdová, Eva - Brzobohatá, Kristýna - Chocholová, Eva - Fialová, Dana - Šmerda, Jaromír
PY  - 2021
TI  - Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing
JF  - Journal of Archaeological Science: Reports
VL  - 40
IS  - December
SP  - 1-5
EP  - 1-5
PB  - Elsevier Ltd.
SN  - 2352409X
KW  - ancient DNA
KW  - mitochondrial DNA
KW  - Sanger sequencing
KW  - hypervariable region
UR  - https://doi.org/10.1016/j.jasrep.2021.103216
N2  - Working with ancient DNA is challenging and requires an authentication step. We analyzed eleven badly preserved samples from the Nitra culture with the influence of Únětice culture dated to the 4000 BP Bronze Age. We present estimated mitochondrial haplogroups of four samples based on Sanger sequencing of HVR1 and a part of HVR2. Sanger sequencing has strong limitations in haplogroup prediction, especially when working with ancient DNA. However, Sanger sequencing in this study is featured as a control step to provide us with quality results of mitochondrial DNA and as a screening of present haplogroups. DNA quality estimation is further supported by sample concentration and fragment analysis. The presence of mitochondrial DNA is confirmed by PCR. However, the Sanger sequencing of mitochondrial DNA can confirm its presence in good quality for further sequencing. Therefore, only suitable samples were selected for further high-throughput sequencing of the whole mitogenome.
ER  -

ŠENOVSKÁ, Anna; Eva DROZDOVÁ; Kristýna BRZOBOHATÁ; Eva CHOCHOLOVÁ; Dana FIALOVÁ a Jaromír ŠMERDA. Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing. \textit{Journal of Archaeological Science: Reports}. Elsevier Ltd., 2021, roč.~40, December, s.~1-5. ISSN~2352-409X. Dostupné z: https://doi.org/10.1016/j.jasrep.2021.103216.

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