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@proceedings{1805127,
author = {Hiriyur Nagaraj, Pradeep and Rasouly, Aviram and Nudler, Evgeny and Korostelev, Andrei and Demo, Gabriel},
booktitle = {XXVI. Annual Congress of Czech and Slovak Societies for Biochemistry and Molecular Biology},
keywords = {transcription translation ribosome RNA polymerase cryoEM},
language = {eng},
isbn = {978-80-907779-1-0},
title = {Transcription-translation coupling at the stages of translation initiation and elongation},
url = {https://csbmb2021.cz/},
year = {2021}
}
TY - CONF
ID - 1805127
AU - Hiriyur Nagaraj, Pradeep - Rasouly, Aviram - Nudler, Evgeny - Korostelev, Andrei - Demo, Gabriel
PY - 2021
TI - Transcription-translation coupling at the stages of translation initiation and elongation
SN - 9788090777910
KW - transcription translation ribosome RNA polymerase cryoEM
UR - https://csbmb2021.cz/
N2 - In Bacteria and Archaea, mRNAs are translated by ribosomes simultaneously as they are transcribed. Visualization of actively transcribing RNA polymerase (RNAP) in E.coli by electron microscopy provided clear insight into physically coupled transcription and translation (CTT) mechanism. Additionally, some biochemical studies confirmed the synchronous rates of transcription and translation in E.coli suggesting a regulation function of the leading ribosome on the RNAP propagation which is crucial for optimal gene expression and genome stability. Here, we present cryo-electron microscopy (cryo-EM) structure of E. coli RNAP core bound to the small ribosomal 30S subunit. Alignment of the RNA exit tunnel of RNAP with the Shine-Dalgarno (SD) binding site of 30S subunit suggests a possible loading mechanism of the mRNA onto the 30S subunit at the stage of translation initiation. The recent expressome structures showed RNAP near the mRNA entry tunnel of the trailing ribosome. This region is more than 80 Å away from the binding site observed in 30S-RNAP structure, suggesting a large-scale relocation of RNAP. The 30S-RNAP and expressome structures may therefore represent interactions that occur at different steps of translation, especially during initiation and elongation. Future studies are aimed at investigating the initiation and elongation steps of CTT using time-resolved cryo-EM approach.
ER -
HIRIYUR NAGARAJ, Pradeep, Aviram RASOULY, Evgeny NUDLER, Andrei KOROSTELEV a Gabriel DEMO. Transcription-translation coupling at the stages of translation initiation and elongation. In \textit{XXVI. Annual Congress of Czech and Slovak Societies for Biochemistry and Molecular Biology}. 2021. ISBN~978-80-907779-1-0.
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