Pseurotin D Induces Apoptosis through Targeting Redox Sensitive
Pathways in Human Lymphoid Leukemia Cells

J 2021

Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells

MOSEJOVÁ, Eva; Rebeka BOSNJAKOVIC; Lukáš KUBALA a Ondřej VAŠÍČEK

Základní údaje

Originální název

Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells

Autoři

MOSEJOVÁ, Eva; Rebeka BOSNJAKOVIC; Lukáš KUBALA a Ondřej VAŠÍČEK

Vydání

Antioxidants, Basel, MDPI, 2021, 2076-3921

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.675

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00123034

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

pseurotin D; proliferation; apoptosis; mitochondrial activity; reactive oxygen species; lymphoma

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 11. 2024 13:04, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Chronic lymphocytic leukemia (CLL) is the most prevalent lymphoid malignancy in many geographical regions of the world. Pseurotin D, a secondary metabolite of fungi, represents a group of bioactive natural products with a newly ascribed range of interesting biological activities. The purpose of this study was to bring new insights into the mechanism behind the effects of pseurotin D on MEC-1 cells as a representative CLL cell line, with a particular focus on selected signaling pathways important in the proliferation of cells and targeting mitochondrial metabolism. Our results showed that pseurotin D was able to significantly inhibit the proliferation of MEC-1 cells and arrested them in the G2/M cell cycle phase. In addition, pseurotin D was able to induce apoptosis. We found that all of these effects were associated with a change in mitochondrial membrane potential and the production of mitochondrial reactive oxygen species (ROS). We showed for the first time that pseurotin D suppresses MEC-1 cell proliferation and induces apoptotic cell death via induction of the collapse of the mitochondria respiratory chain and the ROS-related caspase pathway. Our results show the pseurotins family as promising compounds which could serve as a basis for the development of new compounds in the treatment of lymphoma.

Návaznosti

EF16_025/0007381, projekt VaV

Název: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou

Citovat

MOSEJOVÁ, Eva; Rebeka BOSNJAKOVIC; Lukáš KUBALA a Ondřej VAŠÍČEK. Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells. Antioxidants. Basel: MDPI, 2021, roč. 10, č. 10, s. 1-16. ISSN 2076-3921. Dostupné z: https://doi.org/10.3390/antiox10101576.

@article{1806136,
   author = {Mosejová, Eva and Bosnjakovic, Rebeka and Kubala, Lukáš and Vašíček, Ondřej},
   article_location = {Basel},
   article_number = {10},
   doi = {https://doi.org/10.3390/antiox10101576},
   keywords = {pseurotin D; proliferation; apoptosis; mitochondrial activity; reactive oxygen species; lymphoma},
   language = {eng},
   issn = {2076-3921},
   journal = {Antioxidants},
   title = {Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells},
   url = {https://www.mdpi.com/2076-3921/10/10/1576},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1806136
AU  - Mosejová, Eva - Bosnjakovic, Rebeka - Kubala, Lukáš - Vašíček, Ondřej
PY  - 2021
TI  - Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells
JF  - Antioxidants
VL  - 10
IS  - 10
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 20763921
KW  - pseurotin D
KW  - proliferation
KW  - apoptosis
KW  - mitochondrial activity
KW  - reactive oxygen species
KW  - lymphoma
UR  - https://www.mdpi.com/2076-3921/10/10/1576
N2  - Chronic lymphocytic leukemia (CLL) is the most prevalent lymphoid malignancy in many geographical regions of the world. Pseurotin D, a secondary metabolite of fungi, represents a group of bioactive natural products with a newly ascribed range of interesting biological activities. The purpose of this study was to bring new insights into the mechanism behind the effects of pseurotin D on MEC-1 cells as a representative CLL cell line, with a particular focus on selected signaling pathways important in the proliferation of cells and targeting mitochondrial metabolism. Our results showed that pseurotin D was able to significantly inhibit the proliferation of MEC-1 cells and arrested them in the G2/M cell cycle phase. In addition, pseurotin D was able to induce apoptosis. We found that all of these effects were associated with a change in mitochondrial membrane potential and the production of mitochondrial reactive oxygen species (ROS). We showed for the first time that pseurotin D suppresses MEC-1 cell proliferation and induces apoptotic cell death via induction of the collapse of the mitochondria respiratory chain and the ROS-related caspase pathway. Our results show the pseurotins family as promising compounds which could serve as a basis for the development of new compounds in the treatment of lymphoma.
ER  -

MOSEJOVÁ, Eva; Rebeka BOSNJAKOVIC; Lukáš KUBALA a Ondřej VAŠÍČEK. Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells. \textit{Antioxidants}. Basel: MDPI, 2021, roč.~10, č.~10, s.~1-16. ISSN~2076-3921. Dostupné z: https://doi.org/10.3390/antiox10101576.

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