| TURČANOVÁ, Michaela, Martin HRTOŇ, Petr DVOŘÁK, Kamil NOVÁK, Markéta HERMANOVÁ, Zdeněk BEDNAŘÍK, Stanislav POLZER and Jiří BURŠA. Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy. BioMed Research International. London: Hindawi, 2021, vol. 2021, November 2021, p. 1-13. ISSN 2314-6133. Available from: https://dx.doi.org/10.1155/2021/6879765. |
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@article{1807828,
author = {Turčanová, Michaela and Hrtoň, Martin and Dvořák, Petr and Novák, Kamil and Hermanová, Markéta and Bednařík, Zdeněk and Polzer, Stanislav and Burša, Jiří},
article_location = {London},
article_number = {November 2021},
doi = {http://dx.doi.org/10.1155/2021/6879765},
keywords = {Full-Range Optical Imaging; Planar Collagen Fiber; Polarized Light Microscopy},
language = {eng},
issn = {2314-6133},
journal = {BioMed Research International},
title = {Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy},
url = {https://www.hindawi.com/journals/bmri/2021/6879765/},
volume = {2021},
year = {2021}
}
TY - JOUR
ID - 1807828
AU - Turčanová, Michaela - Hrtoň, Martin - Dvořák, Petr - Novák, Kamil - Hermanová, Markéta - Bednařík, Zdeněk - Polzer, Stanislav - Burša, Jiří
PY - 2021
TI - Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy
JF - BioMed Research International
VL - 2021
IS - November 2021
SP - 1-13
EP - 1-13
PB - Hindawi
SN - 23146133
KW - Full-Range Optical Imaging
KW - Planar Collagen Fiber
KW - Polarized Light Microscopy
UR - https://www.hindawi.com/journals/bmri/2021/6879765/
N2 - A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.
ER -
TURČANOVÁ, Michaela, Martin HRTOŇ, Petr DVOŘÁK, Kamil NOVÁK, Markéta HERMANOVÁ, Zdeněk BEDNAŘÍK, Stanislav POLZER and Jiří BURŠA. Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy. \textit{BioMed Research International}. London: Hindawi, 2021, vol.~2021, November 2021, p.~1-13. ISSN~2314-6133. Available from: https://dx.doi.org/10.1155/2021/6879765.
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