Full-Range Optical Imaging of Planar Collagen Fiber Orientation
Using Polarized Light Microscopy

J 2021

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

TURČANOVÁ, Michaela, Martin HRTOŇ, Petr DVOŘÁK, Kamil NOVÁK, Markéta HERMANOVÁ et. al.

Základní údaje

Originální název

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Autoři

TURČANOVÁ, Michaela (203 Česká republika, garant), Martin HRTOŇ (203 Česká republika), Petr DVOŘÁK (203 Česká republika), Kamil NOVÁK (203 Česká republika), Markéta HERMANOVÁ (203 Česká republika, domácí), Zdeněk BEDNAŘÍK (203 Česká republika, domácí), Stanislav POLZER (203 Česká republika) a Jiří BURŠA (203 Česká republika)

Vydání

BioMed Research International, London, Hindawi, 2021, 2314-6133

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30106 Anatomy and morphology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.246

Kód RIV

RIV/00216224:14110/21:00119394

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1155/2021/6879765

UT WoS

000771938700001

Klíčová slova anglicky

Full-Range Optical Imaging; Planar Collagen Fiber; Polarized Light Microscopy

Štítky

14110112, 14110514, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 1. 2023 10:57, Mgr. Tereza Miškechová

Anotace

V originále

A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.

Návaznosti

GA21-21935S, projekt VaV

Název: Výpočtové modelování pulzačního proudění v poddajných trubicích s aplikací na tandemovou stenózu karotidy

Investor: Grantová agentura ČR, Výpočtové modelování pulzačního proudění v poddajných trubicích s aplikací na tandemovou stenózu karotidy

Citovat

TURČANOVÁ, Michaela, Martin HRTOŇ, Petr DVOŘÁK, Kamil NOVÁK, Markéta HERMANOVÁ, Zdeněk BEDNAŘÍK, Stanislav POLZER a Jiří BURŠA. Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy. BioMed Research International. London: Hindawi, 2021, roč. 2021, November 2021, s. 1-13. ISSN 2314-6133. Dostupné z: https://dx.doi.org/10.1155/2021/6879765.

@article{1807828,
   author = {Turčanová, Michaela and Hrtoň, Martin and Dvořák, Petr and Novák, Kamil and Hermanová, Markéta and Bednařík, Zdeněk and Polzer, Stanislav and Burša, Jiří},
   article_location = {London},
   article_number = {November 2021},
   doi = {http://dx.doi.org/10.1155/2021/6879765},
   keywords = {Full-Range Optical Imaging; Planar Collagen Fiber; Polarized Light Microscopy},
   language = {eng},
   issn = {2314-6133},
   journal = {BioMed Research International},
   title = {Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy},
   url = {https://www.hindawi.com/journals/bmri/2021/6879765/},
   volume = {2021},
   year = {2021}
}

TY  - JOUR
ID  - 1807828
AU  - Turčanová, Michaela - Hrtoň, Martin - Dvořák, Petr - Novák, Kamil - Hermanová, Markéta - Bednařík, Zdeněk - Polzer, Stanislav - Burša, Jiří
PY  - 2021
TI  - Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy
JF  - BioMed Research International
VL  - 2021
IS  - November 2021
SP  - 1-13
EP  - 1-13
PB  - Hindawi
SN  - 23146133
KW  - Full-Range Optical Imaging
KW  - Planar Collagen Fiber
KW  - Polarized Light Microscopy
UR  - https://www.hindawi.com/journals/bmri/2021/6879765/
N2  - A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.
ER  -

TURČANOVÁ, Michaela, Martin HRTOŇ, Petr DVOŘÁK, Kamil NOVÁK, Markéta HERMANOVÁ, Zdeněk BEDNAŘÍK, Stanislav POLZER a Jiří BURŠA. Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy. \textit{BioMed Research International}. London: Hindawi, 2021, roč.~2021, November 2021, s.~1-13. ISSN~2314-6133. Dostupné z: https://dx.doi.org/10.1155/2021/6879765.

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