3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic
Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio
Analysis

J 2021

3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis

MALÁ, Miriam, Petra ITTERHEIMOVÁ, Lukáš HOMOLA, Jana VINOHRADSKÁ, Petr KUBÁŇ et. al.

Základní údaje

Originální název

3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis

Autoři

MALÁ, Miriam (203 Česká republika, domácí), Petra ITTERHEIMOVÁ (203 Česká republika, domácí), Lukáš HOMOLA (203 Česká republika, domácí), Jana VINOHRADSKÁ (203 Česká republika, domácí) a Petr KUBÁŇ (203 Česká republika, garant, domácí)

Vydání

Separations, Basel, MDPI, 2021, 2297-8739

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.344

Kód RIV

RIV/00216224:14110/21:00120173

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/separations8120234

UT WoS

000737166500001

Klíčová slova anglicky

cystic fibrosis; sweat sampling; skin-wash; capillary electrophoresis; contactless conductivity detection; ion ratio

Štítky

14110212, 14110318, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 3. 2022 10:16, Mgr. Tereza Miškechová

Anotace

V originále

Sweat chloride analysis is one of the important approaches in cystic fibrosis diagnosis. The commonly used Macroduct method to acquire sweat samples is semi-invasive, time consuming and expensive. Furthermore, this method often fails to collect a sufficient amount of sweat in newborns due to the insufficient sweating rate. In this work, we present a novel, simple, 3D-printed sampling device that is used to collect sweat specimens completely noninvasively in less than one minute. The device has a flow-through channel adjacent to the skin surface, through which 500 µL of deionized water is flushed and the spontaneously formed sweat on the skin in the channel area is washed into a plastic vial. The developed skin-wash procedure is a single step operation, is completely noninvasive and it always produces a sweat specimen. The ions from the skin-wash are subsequently analyzed by capillary electrophoresis with contactless conductivity detection and selected ion ratio (Cl−/K+) or ((Cl− + Na+)/K+) is used as a cut-off value to diagnose cystic fibrosis patients with sensitivity and specificity comparable to the conventional Macroduct method.

Návaznosti

NV18-08-00189, projekt VaV

Název: Nový postup diagnostiky cystické fibrózy založený na iontových poměrech v neinvazivně získaných vzorcích

Investor: Ministerstvo zdravotnictví ČR, Nový postup diagnostiky cystické fibrózy založený na iontových poměrech v neinvazivně získaných vzorcích

Citovat

MALÁ, Miriam, Petra ITTERHEIMOVÁ, Lukáš HOMOLA, Jana VINOHRADSKÁ a Petr KUBÁŇ. 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis. Separations. Basel: MDPI, 2021, roč. 8, č. 12, s. 1-10. ISSN 2297-8739. Dostupné z: https://dx.doi.org/10.3390/separations8120234.

@article{1808458,
   author = {Malá, Miriam and Itterheimová, Petra and Homola, Lukáš and Vinohradská, Jana and Kubáň, Petr},
   article_location = {Basel},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/separations8120234},
   keywords = {cystic fibrosis; sweat sampling; skin-wash; capillary electrophoresis; contactless conductivity detection; ion ratio},
   language = {eng},
   issn = {2297-8739},
   journal = {Separations},
   title = {3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis},
   url = {https://www.mdpi.com/2297-8739/8/12/234#cite},
   volume = {8},
   year = {2021}
}

TY  - JOUR
ID  - 1808458
AU  - Malá, Miriam - Itterheimová, Petra - Homola, Lukáš - Vinohradská, Jana - Kubáň, Petr
PY  - 2021
TI  - 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis
JF  - Separations
VL  - 8
IS  - 12
SP  - 1-10
EP  - 1-10
PB  - MDPI
SN  - 22978739
KW  - cystic fibrosis
KW  - sweat sampling
KW  - skin-wash
KW  - capillary electrophoresis
KW  - contactless conductivity detection
KW  - ion ratio
UR  - https://www.mdpi.com/2297-8739/8/12/234#cite
N2  - Sweat chloride analysis is one of the important approaches in cystic fibrosis diagnosis. The commonly used Macroduct method to acquire sweat samples is semi-invasive, time consuming and expensive. Furthermore, this method often fails to collect a sufficient amount of sweat in newborns due to the insufficient sweating rate. In this work, we present a novel, simple, 3D-printed sampling device that is used to collect sweat specimens completely noninvasively in less than one minute. The device has a flow-through channel adjacent to the skin surface, through which 500 µL of deionized water is flushed and the spontaneously formed sweat on the skin in the channel area is washed into a plastic vial. The developed skin-wash procedure is a single step operation, is completely noninvasive and it always produces a sweat specimen. The ions from the skin-wash are subsequently analyzed by capillary electrophoresis with contactless conductivity detection and selected ion ratio (Cl−/K+) or ((Cl− + Na+)/K+) is used as a cut-off value to diagnose cystic fibrosis patients with sensitivity and specificity comparable to the conventional Macroduct method.
ER  -

MALÁ, Miriam, Petra ITTERHEIMOVÁ, Lukáš HOMOLA, Jana VINOHRADSKÁ a Petr KUBÁŇ. 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis. \textit{Separations}. Basel: MDPI, 2021, roč.~8, č.~12, s.~1-10. ISSN~2297-8739. Dostupné z: https://dx.doi.org/10.3390/separations8120234.

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